[硫化氢对大鼠急性心肌缺血时心肌线粒体损伤的影响]

Liu Fang, Zhang Jian-Xin, Li Lan-Fang, Zhang Qin-Zeng, Ding Yan-Yan, Zhang Xin-Yan

Department of Pharmacology, Hebei Academy of Medical Sciences, Shijiazhuang 050021, China.

Zhongguo Ying Yong Sheng Li Xue Za Zhi. 2011 May;27(2):158-62.

OBJECTIVE

To investigate the effect of hydrogen sulfide (H2S) on mitochondrial function in acute myocardial ischemia in rats.

METHODS

Acute myocardial ischemia models were established by ligating the left anterior descending coronary artery (LADC) of rats. Fourty-eight male SD rats were randomly divided into 6 groups (n = 8): sham operation group, ischemia group, ischemia + sodium hydrosulfide (NaHS) low, middle and high dose groups and ischemia + DL-proparglycine(PPG) group. The ultrastructures of myocardial mitochondria were observed with electron microscope. The content of H2S in plasma and the activity of cystathionine-gamma-lyase (CSE) in myocardial tissue of rats were respectively detected. The swelling and activity of myocardial mitochondria were determined. The activities of ATPase, GSH-Px, SOD and the content of malondial-dehyde (MDA) in myocardial mitochondria of rats were also measured.

RESULTS

Compared with those of the sham operation group, the content of H2S in plasma, the activity of CSE in myocardial tissue and the activity of myocardium mitochondria were significantly decreased. The activities of ATPase, SOD, GSH-Px in myocardial mitochondria were significantly decreased, The content of malondial dehyde(MDA) in myocardial mitochondria and the swelling of mitochondria were distinctly increased in the ischemia group (P < 0.01). Compared with those of the ischemia group, the content of H2S in plasma and the activity of CSE in myocardial tissue were increased, and the activities of mitochondria, ATPase, SOD, and GSH-Px in myocardial mitochondria were significantly increased in ischemia + NaHS low, middle and high-dose groups; the swelling of mitochondria and the content of MDA in myocardial mitochondria were significantly decreased in ischemia + NaHS middle and high-dose groups (P < 0.05 or P < 0.01). The administration of PPG could partially reduce the myocardial protection of hydrogen sulfide (P < 0.05 or P < 0.01).

CONCLUSION

It could be concluded that the administration of hydrogen sulfide could enhance the activities of mitochondrial ATPase, SOD, GSH-Px, decrease the level of mitochondrial lipid peroxidation, and play a protective effect against acute myocardial ischemia.

目的

探讨硫化氢（H₂S）对大鼠急性心肌缺血时线粒体功能的影响。

方法

通过结扎大鼠左冠状动脉前降支（LADC）建立急性心肌缺血模型。48只雄性SD大鼠随机分为6组（n = 8）：假手术组、缺血组、缺血+低、中、高剂量硫氢化钠（NaHS）组和缺血+DL-炔丙基甘氨酸（PPG）组。用电子显微镜观察心肌线粒体超微结构。分别检测大鼠血浆中H₂S含量及心肌组织中胱硫醚-γ-裂解酶（CSE）活性。测定心肌线粒体肿胀程度及活性。还检测大鼠心肌线粒体中ATP酶、谷胱甘肽过氧化物酶（GSH-Px）、超氧化物歧化酶（SOD）活性及丙二醛（MDA）含量。

结果

与假手术组相比，缺血组血浆中H₂S含量、心肌组织中CSE活性及心肌线粒体活性显著降低。心肌线粒体中ATP酶、SOD、GSH-Px活性显著降低，心肌线粒体中丙二醛（MDA）含量及线粒体肿胀程度明显增加（P < 0.01）。与缺血组相比，缺血+低、中、高剂量NaHS组血浆中H₂S含量及心肌组织中CSE活性升高，心肌线粒体活性、ATP酶、SOD及GSH-Px活性显著升高；缺血+中、高剂量NaHS组线粒体肿胀程度及心肌线粒体中MDA含量显著降低（P < 0.05或P < 0.01）。给予PPG可部分降低硫化氢对心肌的保护作用（P < 0.05或P < 0.01）。