IgA 肾病结局中的 NF-kB 表达。
NF-kB expression in IgA nephropathy outcome.
机构信息
Department of Pathology, University of São Paulo, Ribeirão, Preto, Brazil.
出版信息
Dis Markers. 2011;31(1):9-15. doi: 10.3233/DMA-2011-0795.
Some studies have demonstrated the involvement of nuclear factor-kappa B (NF-kB) in the pathogenesis of glomerulonephritis. The aim of our study was twofold: (1) to analyze the prognostic value of NF-kB expression in primary IgA nephropathy (IgAN) and (2) to compare the results of NF-kB expression by immunohistochemistry (IHC) and southwestern histochemistry (SWH). We analyzed 62 patients diagnosed with IgAN from 1987 to 2003. We used monoclonal antibodies to CD68 and mast cell tryptase and polyclonal antibodies to TGF-β1, α-SMA and NF-kB p65. We used SWH for the in situ detection of activated NF-kB. The results showed that NF-kB expression (mainly by SWH) correlated with clinical and histological parameters. An unfavorable clinical course of IgAN was significantly related to tubular NF-kB expression by SWH, but not by IHC. The Kaplan-Meier curves demonstrated that increased NF-kB expression, which was measured by IHC and SWH, decreased renal survival. In conclusion, the increased expression of NF-kB in the tubular area may be a predictive factor for the poor prognosis of patients with IgAN. Compared with IHC, NF-kB expression determined by SWH was correlated with a larger number of parameters of poor disease outcome.
一些研究表明核因子-κB（NF-κB）参与了肾小球肾炎的发病机制。我们的研究目的有两个：（1）分析 NF-κB 在原发性 IgA 肾病（IgAN）中的表达的预后价值，（2）比较免疫组化（IHC）和西南组织化学（SWH）检测 NF-κB 表达的结果。我们分析了 1987 年至 2003 年间诊断为 IgAN 的 62 例患者。我们使用针对 CD68 和肥大细胞胰蛋白酶的单克隆抗体以及针对 TGF-β1、α-SMA 和 NF-κB p65 的多克隆抗体。我们使用 SWH 进行激活的 NF-κB 的原位检测。结果表明，NF-κB 的表达（主要通过 SWH）与临床和组织学参数相关。IgAN 的不良临床病程与 SWH 检测到的肾小管 NF-κB 表达显著相关，但与 IHC 无关。Kaplan-Meier 曲线表明，通过 IHC 和 SWH 测量的 NF-κB 表达增加会降低肾脏的存活率。总之，肾小管区域 NF-κB 的表达增加可能是 IgAN 患者预后不良的预测因素。与 IHC 相比，SWH 确定的 NF-κB 表达与更多不良疾病结局的参数相关。
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