Ma Jinzhu, Cui Yudong, Zhang Jing, Zhu Zhanbo, Piao Fanze
College of Life Science and Biotechnology, Heilongjiang Bayi Agricultural University, Daqing 163319, China.
Sheng Wu Gong Cheng Xue Bao. 2011 Apr;27(4):566-71.
In order to characterize the immunogenicity and immunoprotection of the Staphylococcus aureus (S. aureus) surface Isdb, we amplified Isdb gene from S. aureus Wood46 strain. The isdb gene was subsequently inserted into pET32a(+) vector and the recombinant plasmid was transformed into E. coli strain BL21. The recombinant Isdb was expressed and purified. Then, we immunized mice with the purified recombinant protein. The antibody level was measured by enzyme-linked immunosorbent assay. Finally, immunized mice were challenged with S. aureus strains Wood46 and HLJ23-1. These results showed that isdb gene sequences were highly conserved, and the recombinant Isdb was successfully expressed. The antibody titer in the immunized groups was increased significantly (P < 0.05) compared with the control, the protective rate of Isdb protein inducted by challenge with the two S. aureus stains Wood46 and HLJ23-1 was 62.5% and 75%, respectively. These results showed that the Isdb protein had high immunogenicity and immunoprotective capacity.
为了表征金黄色葡萄球菌（S. aureus）表面蛋白Isdb的免疫原性和免疫保护作用，我们从金黄色葡萄球菌Wood46菌株中扩增了Isdb基因。随后将isdb基因插入pET32a(+)载体，并将重组质粒转化到大肠杆菌BL21菌株中。表达并纯化重组Isdb。然后，我们用纯化的重组蛋白免疫小鼠。通过酶联免疫吸附测定法测量抗体水平。最后，用金黄色葡萄球菌菌株Wood46和HLJ23-1攻击免疫的小鼠。这些结果表明，isdb基因序列高度保守，并且重组Isdb成功表达。与对照组相比，免疫组的抗体滴度显著升高（P < 0.05），用两种金黄色葡萄球菌菌株Wood46和HLJ23-1攻击诱导的Isdb蛋白的保护率分别为62.5%和75%。这些结果表明，Isdb蛋白具有高免疫原性和免疫保护能力。
