Institute of Botany at the Technische Universität Darmstadt, Schnittspahnstrasse 3-5, 64287 Darmstadt, Germany.
Plant J. 2011 Dec;68(6):977-86. doi: 10.1111/j.1365-313X.2011.04748.x. Epub 2011 Oct 10.
Chlorella viruses are a source of interesting membrane transport proteins. Here we examine a putative K(+) transporter encoded by virus FR483 and related chlorella viruses. The protein shares sequence and structural features with HAK/KUP/KT-like K(+) transporters from plants, bacteria and fungi. Yeast complementation assays and Rb(+) uptake experiments show that the viral protein, termed HAKCV (high-affinity K(+) transporter of chlorella virus), is functional, with transport characteristics that are similar to those of known K(+) transporters. Expression studies revealed that the protein is expressed as an early gene during viral replication, and proteomics data indicate that it is not packaged in the virion. The function of HAKCV is unclear, but the data refute the hypothesis that the transporter acts as a substitute for viral-encoded K(+) channels during virus infection.
绿藻病毒是一类有趣的膜转运蛋白的来源。在这里，我们研究了一种由 FR483 病毒和相关绿藻病毒编码的假定的 K(+)转运蛋白。该蛋白与来自植物、细菌和真菌的 HAK/KUP/KT 样 K(+)转运蛋白在序列和结构上具有相似性。酵母互补实验和 Rb(+)摄取实验表明，该病毒蛋白被称为 HAKCV（绿藻病毒的高亲和性 K(+)转运蛋白），是具有功能的，其转运特性与已知的 K(+)转运蛋白相似。表达研究表明，该蛋白在病毒复制过程中作为早期基因表达，蛋白质组学数据表明它不被包装在病毒粒子中。HAKCV 的功能尚不清楚，但这些数据反驳了转运蛋白在病毒感染过程中作为病毒编码的 K(+)通道替代物的假说。
