Chayen J, Pitsillides A A, Bitensky L, Muir I H, Taylor P M, Askonas B A
Division of Cellular Biology, Mathilda and Terence Kennedy Institute of Rheumatology, London, UK.
J Exp Pathol (Oxford). 1990 Apr;71(2):197-208.
The mechanism by which cytotoxic T-lymphocytes (Tc) induce the death of specific target cells is still controversial. We have used quantitative cytochemical methods to distinguish the metabolic activities of the target cells from those of the Tc, even when they are attached to each other. Early events following Tc-P8(15) target cell interaction were first, increased glucose 6-phosphate dehydrogenase activity and second, labilization of the lysosomes within the target cell: these changes could be mimicked, in part, by polyamines and could be inhibited by inhibiting ornithine decarboxylase (ODC) activity. The crucial role of ODC in the chain of events that led to cytolysis in this particular experimental system was shown first, by measuring ODC activity directly and secondly, by the inhibition of cytolysis by the presence of a selective inhibitor of ODC activity.
细胞毒性T淋巴细胞（Tc）诱导特定靶细胞死亡的机制仍存在争议。我们使用定量细胞化学方法来区分靶细胞和Tc的代谢活性，即使它们相互附着。Tc与P8(15)靶细胞相互作用后的早期事件，首先是葡萄糖6-磷酸脱氢酶活性增加，其次是靶细胞内溶酶体的不稳定：这些变化部分可被多胺模拟，并可通过抑制鸟氨酸脱羧酶（ODC）活性来抑制。在这个特定的实验系统中，ODC在导致细胞溶解的事件链中的关键作用首先通过直接测量ODC活性得以体现，其次通过ODC活性选择性抑制剂的存在对细胞溶解的抑制得以证明。
