Ge Qing-fang, Zhang Hui-yun
Key Laboratory of Classics Theory of Traditional Chinese Medicine of the Ministry of Education of China, Shandong University of Traditional Chinese Medicine, Jinan 250355, China.
Zhong Xi Yi Jie He Xue Bao. 2011 Aug;9(8):871-7. doi: 10.3736/jcim20110809.
To explore the central mechanisms of anger emotion and the effects of Chinese herbal medicines for regulating liver qi on the anger emotion and the expression level of 5-hydroxytryptamine 3B receptor (5-HT3BR) in rat hypothalamus.
Rat models of anger-in or anger-out emotions were prepared by the methods of resident intruder paradigm. There were five groups in this study: control, anger-in model, Jingqianshu Granule-treated anger-in, anger-out model and Jingqianping Granule-treated anger-out groups. The treatment groups were orally given Jingqianshu granules and Jingqianping granules respectively, and the model groups and the normal control group were given sterile water. Open-field test and sucrose preference test were used to evaluate behavioristics of the rats. Semi-quantitative reverse transcription-polymerase chain reaction and Western blot methods were used to detect the expression levels of 5-HT3BR mRNA and protein in the rat hypothalamus.
The expression of 5-HT3BR in hypothalamus of anger-in model rats increased obviously (P<0.01) and that of anger-out model rats decreased obviously (P<0.01) compared with the normal control group. Compared with the model group, the expressions of 5-HT3BR in the treatment groups were significantly improved (P<0.01) after treatment, and recovered to normal level.
The anger-in stimulation obviously increases hypothalamic 5-HT3BR expression and the anger-out emotion can obviously reduce its expression. Chinese herbal medicines for regulating liver qi may treat anger emotion in rats by improving the hypothalamic 5-HT3BR protein and gene expression levels.
探讨愤怒情绪的中枢机制以及疏肝理气中药对大鼠愤怒情绪及下丘脑5-羟色胺3B受体（5-HT3BR）表达水平的影响。
采用定居者-入侵者范式制备愤怒郁积或愤怒爆发情绪的大鼠模型。本研究共分为五组：对照组、愤怒郁积模型组、经荆前舒颗粒治疗的愤怒郁积组、愤怒爆发模型组和经荆前平颗粒治疗的愤怒爆发组。治疗组分别口服荆前舒颗粒和荆前平颗粒，模型组和正常对照组给予无菌水。采用旷场试验和蔗糖偏好试验评估大鼠的行为学表现。采用半定量逆转录-聚合酶链反应和蛋白质印迹法检测大鼠下丘脑5-HT3BR mRNA和蛋白的表达水平。
与正常对照组相比，愤怒郁积模型大鼠下丘脑5-HT3BR的表达明显增加（P<0.01），愤怒爆发模型大鼠下丘脑5-HT3BR的表达明显降低（P<0.01）。与模型组相比，治疗组治疗后5-HT3BR的表达明显改善（P<0.01），并恢复至正常水平。
愤怒郁积刺激明显增加下丘脑5-HT3BR的表达，愤怒爆发情绪可明显降低其表达。疏肝理气中药可能通过提高下丘脑5-HT3BR蛋白和基因表达水平来治疗大鼠的愤怒情绪。
