Yao Xiao-Li, Liu Qiang, Ye Cheng-Hui, Li Zhi-Ping, Lu Xi-Lin, Li Pan-Long, Li Xiao-Bo, Li Wei-Qiang
Department of Neurology, The First Affiliated Hospital, Sun Yat-Sen University, Guangzhou, People's Republic of China.
Neuroreport. 2011 Oct 5;22(14):689-95. doi: 10.1097/WNR.0b013e32834a280a.
Induced pluripotent stem (iPS) cells have been generated from somatic cells by ectopic expression of defined transcription factors. The important issues for clinical applications of iPS cells are the defined methods for somatic cell differentiation and how to effectively enrich desired cell population. Here we used humanized renilla green fluorescent protein under the control of Tα1 α-tubulin promoter as lineage selection marker for neuronal differentiation of iPS cells. Using fluorescence-activated cell sorting, green fluorescent protein positive cells were isolated and enriched to near-purity. These results indicated that the neuronal differentiation potential of iPS cells derived from adult somatic cells is similar to that of embryonic stem cells and the high-purity neurons may have important implications for neurodevelopmental studies, safety pharmacological studies, and transplantation studies.
通过异位表达特定转录因子，已从体细胞中生成了诱导多能干细胞（iPS细胞）。iPS细胞临床应用的重要问题是体细胞分化的明确方法以及如何有效富集所需细胞群体。在此，我们使用受Tα1α-微管蛋白启动子控制的人源化海肾绿色荧光蛋白作为iPS细胞神经元分化的谱系选择标记。通过荧光激活细胞分选，分离并富集绿色荧光蛋白阳性细胞至接近纯品状态。这些结果表明，源自成人体细胞的iPS细胞的神经元分化潜能与胚胎干细胞相似，高纯度神经元可能对神经发育研究、安全药理学研究和移植研究具有重要意义。
