Department of Obstetric and Gynecology, Shengjing Hospital of the China Medical University Shenyang, Shenyang 110004, China.
J Cancer Res Clin Oncol. 2011 Nov;137(11):1721-7. doi: 10.1007/s00432-011-1019-8. Epub 2011 Aug 18.
To determine the correlations of human papillomavirus (HPV) infection and α6 integrin up-regulation to the tumorigenesis and development of cervical cancer, and develop an on-chip antibody-based detection system using the microelectromechanical system (MEMS) device for recognizing and capturing cervical cancer cells from a mixed cell population.
We determined the efficiency of an antibody-based MEMS platform in recognizing and capturing cervical cancer cells. In addition, α6 integrin was used as a capture antibody bound to the channel surface. Channels 2 cm long × 50 cm wide with inlet and outlet diameters of 50 cm were formed in microfluidic polydimethylsiloxane (PDMS) chips.
The overall chip measured 2 cm × 1.5 cm × 0.5 cm. Using a syringe micropump, 20,000 normal human cervical epithelial cells (HCEC), 20,000 human cervical stromal cells (HCSC), and 20,000 human cervical cancer cells (HCCC) were suspended in PBS and flowed through the system at a rate of 20 mL/min. At this flow rate, more than 45% of cancer cells were captured, whereas less than 5% of normal cells were captured. In addition, we confirmed the specificity of this system in cell-type targeting using PCR.
MEMS is a sensitive and accurate method for capturing and enriching cells of interest. This technique is potentially useful in detecting cervical cancer at all stages, as well as other cancers with similar characteristics of cell surface antigen expression.
确定人乳头瘤病毒（HPV）感染和α6 整合素上调与宫颈癌发生和发展的相关性，并利用微机电系统（MEMS）装置开发一种基于抗体的芯片检测系统，用于从混合细胞群中识别和捕获宫颈癌细胞。
我们确定了基于抗体的 MEMS 平台识别和捕获宫颈癌细胞的效率。此外，α6 整合素被用作结合在通道表面的捕获抗体。在微流控聚二甲基硅氧烷（PDMS）芯片中形成长 2 厘米、宽 50 厘米、进口和出口直径为 50 厘米的通道。
整个芯片尺寸为 2 厘米×1.5 厘米×0.5 厘米。使用注射器微量泵，以 20,000 个正常人类宫颈上皮细胞（HCEC）、20,000 个人类宫颈基质细胞（HCSC）和 20,000 个人类宫颈癌细胞（HCCC）悬浮在 PBS 中，以 20 毫升/分钟的速度流过系统。在这个流速下，超过 45%的癌细胞被捕获，而不到 5%的正常细胞被捕获。此外，我们使用 PCR 证实了该系统在细胞类型靶向方面的特异性。
MEMS 是一种敏感且准确的捕获和富集感兴趣细胞的方法。该技术在检测所有阶段的宫颈癌以及其他具有类似细胞表面抗原表达特征的癌症方面具有潜在的应用价值。
