Ye Feng, Yang Yi-Xuan, Hu Huai-Dong, Tong Jian-Bin, Cai Wei-Jun, Zhang Da-Zhi, Hu Peng, Li Sang-Lin, Ren Hong
Key Laboratory of Molecular Biology for Infectious Diseases, Ministry of Education of China, The Second Affiliated Hospital, Chongqing Medical University, Chongqing, China.
Hybridoma (Larchmt). 2011 Aug;30(4):393-5. doi: 10.1089/hyb.2011.0034.
Ski is an avian sarcoma virus oncogene homolog best known for inhibiting TGF beta signaling through its association with the SMAD proteins. Anti-Ski antibodies (MAbs) of high titer were prepared by immunizing BALB/c mice with multifocal intradermal injections and fusing high titer antibody producing spleen cells with myeloma cells of SP2/0 origin. Three MAbs were selected for further characterization as classes and subclasses. Antibodies were produced by these three clones with high affinities ranging from 10(9) to 10(11)/m. These clones were found to be of the immunoglobulin IgG1 and IgG2b subclass with kappa light chain. They could recognize Ski as determined by Western blot analysis. The produced MAbs will be a useful tool for further investigation of Ski functions in organisms.
Ski是一种禽肉瘤病毒癌基因同源物，因其与SMAD蛋白结合抑制转化生长因子β（TGFβ）信号传导而闻名。通过多焦点皮内注射免疫BALB/c小鼠，并将产生高滴度抗体的脾细胞与源自SP2/0的骨髓瘤细胞融合，制备了高滴度的抗Ski单克隆抗体（MAbs）。选择三种MAbs进一步鉴定其类别和亚类。这三个克隆产生的抗体具有10(9)至10(11)/m的高亲和力。发现这些克隆属于带有κ轻链的免疫球蛋白IgG1和IgG2b亚类。通过蛋白质印迹分析确定它们可以识别Ski。所产生的MAbs将成为进一步研究Ski在生物体中功能的有用工具。
