Kim Hana, Choi Hong Seo, Kim Cheorl-Ho, Ryu Chun Jeih
Insitute of Bioscience, Department of Bioscience and Biotechnology, Sejong University, 98 Gunja-Dong, Gwangjin-Gu, Seoul, Korea.
Hybridoma (Larchmt). 2011 Aug;30(4):401-4. doi: 10.1089/hyb.2011.0020.
To investigate cell surface antigens on human embryonic stem cells (hESCs), we generated a panel of monoclonal antibodies (MAbs) against undifferentiated hESCs by a decoy immunization strategy. One of the MAbs, MAb 2-E2, specifically bound to human pluripotent stem cells but not to mouse pluripotent stem cells and mouse embryonic fibroblasts. 2-E2 also bound to human differentiated cells, peripheral blood monocytes, and dermal fibroblasts. 2-E2 antigen expression drastically decreased in retinoic acid-induced differentiated hESCs. However, it gradually increased after initial decrease during embryoid body formation of hESCs. 2-E2 recognized approximately 68 and 27 kDa proteins present on the surface of human pluripotent stem cells. The results suggest that 2-E2-specific surface protein is a novel cell surface protein that plays a role in the early differentiation of human pluripotent stem cells.
为了研究人类胚胎干细胞（hESCs）的细胞表面抗原，我们通过诱饵免疫策略制备了一组针对未分化hESCs的单克隆抗体（MAbs）。其中一种单克隆抗体，即MAb 2-E2，特异性地结合人类多能干细胞，但不结合小鼠多能干细胞和小鼠胚胎成纤维细胞。2-E2也结合人类分化细胞、外周血单核细胞和真皮成纤维细胞。在视黄酸诱导分化的hESCs中，2-E2抗原表达急剧下降。然而，在hESCs形成胚状体的过程中，它在最初下降后逐渐增加。2-E2识别出存在于人类多能干细胞表面的大约68和27 kDa的蛋白质。结果表明，2-E2特异性表面蛋白是一种新型细胞表面蛋白，在人类多能干细胞的早期分化中发挥作用。
