从大肠杆菌中克隆溴化乙锭外排基因。
Cloning of the ethidium efflux gene from Escherichia coli.
作者信息
Purewal A S, Jones I G, Midgley M
机构信息
Department of Applied Biology, University of Hull, U.K.
出版信息
FEMS Microbiol Lett. 1990 Mar 1;56(1-2):73-6. doi: 10.1016/0378-1097(90)90127-c.
PMID:2185131
Abstract
The gene specifying the ethidium efflux system of Escherichia coli has been cloned on a 3.2 kbp HindIII fragment and located on a 1.2 kbp fragment within this. Cross-resistance studies indicate that the system has a broad specificity for monovalent cations and the gene shows no hybridisation with similar genes found in Staphylococci.
摘要
编码大肠杆菌溴化乙锭外排系统的基因已被克隆到一个3.2千碱基对的HindIII片段上，并定位在其中一个1.2千碱基对的片段上。交叉耐药性研究表明，该系统对单价阳离子具有广泛的特异性，并且该基因与葡萄球菌中发现的类似基因没有杂交现象。
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