Laboratorio de Biotecnología y Virología Vegetal, Centro de Microbiología y Biología Celular, Instituto Venezolano de Investigaciones Científicas, Caracas, Venezuela.
Arch Virol. 2011 Dec;156(12):2263-6. doi: 10.1007/s00705-011-1093-x. Epub 2011 Aug 19.
Virus isolate T217L was obtained from a diseased tomato (Solanum lycopersicum) plant showing leaf deformation and chlorotic mottle symptoms near Maracaibo in the state of Zulia, Venezuela. Full-length DNA-A and DNA-B molecules of T217L were cloned and sequenced. The genome organization of T217L was identical to the bipartite genomes of other begomoviruses described from the Americas. Characteristic disease symptoms were reproduced in S. lycopersicum and Capsicum annum plants inoculated using the cloned viral DNA-A and DNA-B components, confirming disease aetiology. A sequence analysis of DNA-A showed that the T217L isolate has the highest sequence identity (84%) with sida yellow mosaic Yucatan virus (SiYMYuV), sida golden mosaic Honduras virus (SiGMHV) and bean dwarf mosaic virus (BDMV) isolates. This is less than the 89% identity in the DNA-A component that has been defined as the threshold value for the demarcation of species in the genus Begomovirus. The molecular data show that isolate T217L belongs to a novel tentative begomovirus species, for which the name tomato chlorotic leaf distortion virus is proposed. TCLDV was also detected in symptomatic C. chinense plants growing near the T217L-infected plant.
病毒分离物 T217L 是从委内瑞拉苏利亚州马拉凯附近一株表现叶片变形和褪绿斑驳症状的患病番茄（Solanum lycopersicum）植物中获得的。T217L 的全长 DNA-A 和 DNA-B 分子被克隆和测序。T217L 的基因组组织与从美洲描述的其他双组分伴生病毒的基因组相同。使用克隆的病毒 DNA-A 和 DNA-B 成分接种番茄和辣椒植物，重现了典型的疾病症状，证实了病因。DNA-A 的序列分析表明，T217L 分离物与西印度菘蓝黄花叶病毒（SiYMYuV）、中美洲金叶斑病毒（SiGMHV）和菜豆矮化花叶病毒（BDMV）分离物具有最高的序列同一性（84%）。这低于双生病毒属中定义为物种划分界限的 DNA-A 成分的 89%同一性。分子数据表明，分离物 T217L 属于一种新的暂定伴生病毒种，建议将其命名为番茄褪绿斑曲叶病毒。在感染 T217L 的植物附近生长的有症状的 C. chinense 植物中也检测到了 TCLDV。
