Molchanov A Iu, Ivanovskaia M G, Rapoport S I, Molchanova E S
Klin Lab Diagn. 2011 Jun(6):3-7.
The review considers main approaches to solving the problem in the quantification of melatonin and describes physicochemical and biological methods for melatonin determination in biological fluids (saliva, urine, plasma) and tissues. The impetus to writing this paper came from the considerably growing interest of the scientific community in melatonin and the in-depth studies of its physiological role, which had revealed a diversity of its previously unknown most important functions and confirmed its presence in many compartments of the body. Due to the specificity of its molecular structure and low concentration, melatonin remains invisible for many conventional analyses. The way out may be combination detection that involves the benefits of a few procedures and that has emerged at the interface of various sciences.
本综述探讨了褪黑素定量分析中解决该问题的主要方法，并描述了用于测定生物体液（唾液、尿液、血浆）和组织中褪黑素的物理化学及生物学方法。撰写本文的契机源于科学界对褪黑素的兴趣大幅增长，以及对其生理作用的深入研究，这些研究揭示了其众多此前未知的重要功能，并证实了它在人体许多部位的存在。由于其分子结构的特殊性和低浓度，褪黑素在许多传统分析中难以被检测到。解决办法可能是采用组合检测，这种方法融合了多种检测程序的优点，是在多学科交叉领域中发展起来的。
