Institute of Dental Sciences, Hebrew University-Hadassah School of Dental Medicine, PO Box 122722, Jerusalem 91120, Israel.
Expert Rev Vaccines. 2011 Aug;10(8):1169-82. doi: 10.1586/erv.11.66.
Plasmid DNA is a promising vaccine modality that is regularly examined in prime-boost immunization regimens. Recent advances in skin immunity increased our understanding of the sophisticated cutaneous immune network, which revived scientific interest in delivering vaccines to the skin. Intradermal administration of plasmid DNA via needle injection is a simple and inexpensive procedure that exposes the plasmid and its encoded antigen to the dermal immune surveillance system. This triggers unique mechanisms for eliciting local and systemic immunity that can confer protection against pathogens and tumors. Understanding the mechanisms of intradermal plasmid DNA immunization is essential for enhancing and modulating its immunogenicity. With regard to vaccination, this is of greater importance as this routine injection technique is highly desirable for worldwide immunization. This article will focus on the current understanding of the mechanisms involved in antigen expression and presentation during primary and secondary syringe and needle intradermal plasmid DNA immunization.
质粒 DNA 是一种很有前途的疫苗模式，经常在初级-加强免疫方案中进行检查。皮肤免疫的最新进展增加了我们对复杂的皮肤免疫网络的理解，这重新激发了将疫苗递送到皮肤的科学兴趣。通过针注射进行皮内给予质粒 DNA 是一种简单且廉价的程序，它使质粒及其编码的抗原暴露于真皮免疫监视系统。这引发了独特的机制，引起局部和全身免疫，可以提供针对病原体和肿瘤的保护。了解皮内质粒 DNA 免疫的机制对于增强和调节其免疫原性至关重要。就疫苗接种而言，这更为重要，因为这种常规注射技术在全球免疫接种中非常理想。本文将重点介绍在初级和次级注射器和针皮内质粒 DNA 免疫接种过程中抗原表达和呈递所涉及的机制的最新理解。
