van den Berg Joost H, Oosterhuis Koen, Schumacher Ton N M, Haanen John B A G, Bins Adriaan D
Division of Immunology, The Netherlands Cancer Institute, Plesmanlaan 121, 1066 CX, Amsterdam, The Netherlands.
Methods Mol Biol. 2014;1143:131-40. doi: 10.1007/978-1-4939-0410-5_9.
DNA vaccination is an attractive vaccination method. First, the production of plasmid DNA as a vaccine is considerably more cheap and simple than the production of recombinant protein. Second, the expression cassette of DNA vaccines can readily be modified, making DNA vaccines highly flexible. Finally, in animal models, DNA vaccination is able to induce potent cellular immune responses. Over the past decade, the focus in the DNA vaccination field has in large part moved from intramuscular immunization towards dermal administration. As a natural "porte d'entrée" for pathogens, the skin is rich in antigen-presenting cells, which are required for generating an efficient antigen-specific immune response. This chapter describes a DNA vaccination protocol that utilizes a simple tattooing device for the dermal delivery of plasmid DNA. This technique, called DNA tattooing, is capable of generating high frequencies of antigen-reactive T cells in mice and macaques.
DNA疫苗接种是一种颇具吸引力的疫苗接种方法。首先，作为疫苗的质粒DNA的生产比重组蛋白的生产要便宜得多且简单得多。其次，DNA疫苗的表达盒可以很容易地进行修饰，这使得DNA疫苗具有高度的灵活性。最后，在动物模型中，DNA疫苗接种能够诱导强烈的细胞免疫反应。在过去十年中，DNA疫苗接种领域的重点在很大程度上已从肌肉注射免疫转向经皮给药。作为病原体的天然“入口”，皮肤富含抗原呈递细胞，而这些细胞是产生有效的抗原特异性免疫反应所必需的。本章描述了一种DNA疫苗接种方案，该方案利用一种简单的纹身装置经皮递送质粒DNA。这种技术称为DNA纹身，能够在小鼠和猕猴中产生高频率的抗原反应性T细胞。
