Office of Regulatory Activities, National Center for Toxicological Research, Jefferson, AR 72079, United States.
Biochem Biophys Res Commun. 2011 Nov 18;415(2):220-3. doi: 10.1016/j.bbrc.2011.07.115. Epub 2011 Aug 11.
Polymorphic C-to-T change in the promoter region of DNA-methyltransferase-3B (DNMT3B) gene is associated with risk of several cancers. The aim of this study was to investigate the effect of DNMT3B promoter genetic variant on its transcriptional activity and to compare activity in several pancreatic cell lines. DNMT3B promoter constructs carrying either -149C allele or -149T allele were transiently transfected into pancreatic cancer cells. In promoter assaying, carriage of -149T allele showed only a slight activity (1.1-fold) in Mia cells (p=0.462). In contrast, significant increase (3.8-fold) in activity of -149T allele was shown in SU86.86 pancreatic cancer cells (p=0.0001). These preliminary findings suggest that genetic variance may influence DNMT3B expression in pancreatic cancer. Further studies are needed.
DNA 甲基转移酶-3B（DNMT3B）基因启动子区的多态性 C 到 T 变化与多种癌症的风险相关。本研究旨在研究 DNMT3B 启动子遗传变异对其转录活性的影响，并比较几种胰腺细胞系中的活性。携带 -149C 等位基因或 -149T 等位基因的 DNMT3B 启动子构建体被瞬时转染到胰腺癌细胞中。在启动子测定中，-149T 等位基因在 Mia 细胞中的活性仅略有增加（1.1 倍）（p=0.462）。相比之下，-149T 等位基因在 SU86.86 胰腺癌细胞中的活性显著增加（3.8 倍）（p=0.0001）。这些初步发现表明遗传变异可能影响胰腺癌中的 DNMT3B 表达。需要进一步研究。
