Faculty of Pharmacy, Pharmaceutical Analytical Chemistry Department, Zagazig University, Egypt.
J Chromatogr A. 2011 Sep 23;1218(38):6727-31. doi: 10.1016/j.chroma.2011.07.090. Epub 2011 Aug 6.
In this study, a method for enantioseparation of terbutaline and salbutamol was established using Chirobiotic V column as a stationary phase. Polar ionic mode applying mobile phase containing ammonium nitrate in 100% ethanol, pH 5.1 was found to give the best separation. The salt concentration in the mobile phase and pH value were found to be the most important chromatographic factors affecting separation. Separation of enantiomers of these two basic analytes was complete in less than 10 min without applying ammonium trifluoroacetate (ATFA) or triethylamine (TEA) salts.
本研究建立了一种使用 Chirobiotic V 柱作为固定相对手特布他林和沙丁胺醇对映体进行拆分的方法。发现采用极性离子模式，以含 100%乙醇的硝酸铵作为流动相，pH 值为 5.1 时，分离效果最佳。流动相中盐浓度和 pH 值是影响分离的最重要的色谱因素。在不使用三氟乙酸铵（ATFA）或三乙胺（TEA）盐的情况下，这两种碱性分析物的对映体在不到 10 分钟内即可完全分离。
