Department of Life Sciences, Bhavnagar University, Bhavnagar, Gujarat, India.
Bioresour Technol. 2011 Oct;102(20):9668-74. doi: 10.1016/j.biortech.2011.07.069. Epub 2011 Jul 26.
Degradation of chrysene, a four ring High Molecular Weight (HMW) Polycyclic Aromatic Hydrocarbon (PAH) is of intense environmental interest, being carcinogenic, teratogenic and mutagenic. Multiple PAH degrading halotolerant Achromobacter xylosoxidans was isolated from crude oil polluted saline site. Response Surface Methodology (RSM) using Central Composite Design (CCD) of Bushnell-Haas medium components was successfully employed for optimization resulting 40.79% chrysene degradation on 4th day. The interactions between variables as chrysene and glucose concentrations, pH and inoculum size on degradation were examined by RSM. Under optimum conditions, A. xylosoxidans exhibited 85.96% chrysene degradation on 5th day. The optimum values predicted by RSM were confirmed through confirmatory experiments. It was also noted that pH and glucose as co-substrate play a dynamic role in enhancement of chrysene degradation. Hence, A. xylosoxidans can be further used for subsequent microcosm and in situ experiments for its potential to remediate PAH contaminated saline and non-saline soils.
降解屈，一种四环高分子量（HMW）多环芳烃（PAH），具有强烈的环境意义，具有致癌性、致畸性和致突变性。从受原油污染的盐碱地中分离出了耐盐性多环芳烃降解菌阿克曼氏菌（Achromobacter xylosoxidans）。Bushnell-Haas 培养基成分的中心组合设计（CCD）响应面法（RSM）成功地用于优化，第 4 天的屈降解率达到 40.79%。RSM 研究了变量之间的相互作用，如屈和葡萄糖浓度、pH 值和接种量对降解的影响。在最佳条件下，阿克曼氏菌在第 5 天的屈降解率达到 85.96%。通过验证实验证实了 RSM 预测的最佳值。还注意到，pH 值和葡萄糖作为共底物在增强屈降解方面发挥了动态作用。因此，阿克曼氏菌可以进一步用于随后的微宇宙和原位实验，以评估其修复受多环芳烃污染的盐碱地和非盐碱地土壤的潜力。
