Laboratoire de Biologie Cellulaire et Moléculaire (équipe de Microbiologie), Université des Sciences et de la Technologie, Houari Boumédiènne, Bab Ezzouar, Algiers, Algeria.
Laboratoire de Microbiologie MIO-IRD, UMR 235, Aix-Marseille Université, case 925, 163 Avenue de Luminy, 13288 Marseille cedex 9, France.
Int J Syst Evol Microbiol. 2012 Jul;62(Pt 7):1491-1498. doi: 10.1099/ijs.0.028985-0. Epub 2011 Aug 19.
A novel filamentous bacterium, designated NariEX(T), was isolated from soil collected from Chott Melghir salt lake, which is located in the south-east of Algeria. The strain was an aerobic, halotolerant, thermotolerant, Gram-positive bacterium that was able to grow in NaCl concentrations up to 21% (w/v), at 37-60 °C and at pH 5.0-9.5. The major fatty acids were iso- and anteiso-C(15:0). The DNA G+C content was 47.3 mol%. The major menaquinone was MK-7, but MK-6 and MK-8 were also present. The polar lipid profile consisted of phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylmonomethylethanolamine (methyl-PE). Results of molecular and phenotypic analysis led to the description of the strain as a new member of the family Thermoactinomycetaceae. The isolate was distinct from members of recognized genera of this family by morphological, biochemical and chemotaxonomic characteristics. Strain NariEX(T) showed 16S rRNA gene sequence similarities of 95.38 and 94.28% with the type strains of Desmospora activa and Kroppenstedtia eburnea, respectively, but differed from both type strains in its sugars, polar lipids and in the presence of methyl-PE. On the basis of physiological and phylogenetic data, strain NariEX(T) represents a novel species of a new genus of the family Thermoactinomycetaceae for which the name Melghirimyces algeriensis gen. nov., sp. nov. is proposed. The type strain of Melghirimyces algeriensis, the type species of the genus, is NariEX(T) (=DSM 45474(T)=CCUG 59620(T)).
一株新型丝状细菌，命名为 NariEX(T)，从位于阿尔及利亚东南部的 Chott Melghir 盐湖土壤中分离得到。该菌株为需氧、嗜盐、耐热的革兰氏阳性菌，能够在 21%（w/v）的 NaCl 浓度下、37-60°C 和 pH5.0-9.5 的条件下生长。主要脂肪酸为异和anteiso-C(15:0)。DNA G+C 含量为 47.3 mol%。主要的menaquinone 是 MK-7，但也存在 MK-6 和 MK-8。极性脂谱由磷脂酰甘油、双磷脂酰甘油、磷脂乙醇胺和磷脂单甲乙醇胺（甲基-PE）组成。分子和表型分析的结果表明，该菌株是 Thermoactinomycetaceae 家族的一个新成员。该分离株与该家族中公认属的成员在形态学、生物化学和化学分类学特征上有明显不同。菌株 NariEX(T)与 Desmospora activa 和 Kroppenstedtia eburnea 的模式菌株的 16S rRNA 基因序列相似性分别为 95.38%和 94.28%，但在糖、极性脂和存在甲基-PE 方面与两种模式菌株均不同。基于生理和系统发育数据，菌株 NariEX(T)代表了 Thermoactinomycetaceae 家族的一个新属的一个新种，提议建立一个新属 Melghirimyces algeriensis gen. nov.，sp. nov.。Melghirimyces algeriensis 的模式菌株，即该属的模式种，是 NariEX(T)=DSM 45474(T)=CCUG 59620(T)。
