Ozkan N, Salva E, Cakalağaoğlu F, Tüzüner B
Marmara University, Vocational Health School, Pathology Laboratory, Istanbul, Turkey.
Biotech Histochem. 2012 Feb;87(2):148-53. doi: 10.3109/10520295.2011.590155. Epub 2011 Aug 23.
Formalin has long been the standard fixative for clinical routines worldwide. After the Formaldehyde Standard became law in the US in 1987, as a result of increasing concerns about the potential carcinogenicity of formaldehyde, attempts have been made to find safer alternatives. Alcoholic formalin is a useful fixative, because in addition to fixation, dehydration also is begun. For centuries, honey has been known to be an antibacterial agent with the potential to preserve compounds without harmful effects on its users. We compared the effects of honey fixation with other routine fixatives using conventional histochemical and immunohistochemical staining methods. Our results demonstrated that tissues fixed in either honey or alcoholic formalin and 10% neutral buffered formalin (NBF) have similar histomorphology. Honey fixation showed minor histomorphological differences among the various tissues; however, it did not influence affect correct diagnostic conclusions. Our results suggested that honey can be used as a safe alternative to formalin in histopathology.
长期以来，福尔马林一直是全球临床常规使用的标准固定剂。1987年美国甲醛标准成为法律后，由于人们对甲醛潜在致癌性的担忧日益增加，人们一直在尝试寻找更安全的替代品。酒精福尔马林是一种有用的固定剂，因为除了固定作用外，还开始了脱水过程。几个世纪以来，人们都知道蜂蜜是一种抗菌剂，有保存化合物的潜力，且对使用者没有有害影响。我们使用传统的组织化学和免疫组织化学染色方法，比较了蜂蜜固定与其他常规固定剂的效果。我们的结果表明，用蜂蜜、酒精福尔马林或10%中性缓冲福尔马林（NBF）固定的组织具有相似的组织形态学。蜂蜜固定在不同组织之间显示出微小的组织形态学差异；然而，它并不影响正确的诊断结论。我们的结果表明，在组织病理学中，蜂蜜可以作为福尔马林的安全替代品。
