[无机砷对Chang肝细胞中Nrf2及其调控的下游抗氧化酶mRNA表达的影响]
[Effects of inorganic arsenic on mRNA expression of Nrf2 and Nrf2-regulated downstream anti-oxidant enzymes in Chang hepatocytes].
作者信息
Li Bing, Li Xin, Zhang Xinyu, Zhu Bo, Liu Dan, Xing Xiaoyue, Wang Xin, Ma Ying, Sun Guifan
机构信息
Department of Occupational and Environmental Health, Liaoning Provincial Key Laboratory of Arsenic Biological Effect and Poisoning, School of Public Health, China Medical University, Shenyang 110001, China.
出版信息
Wei Sheng Yan Jiu. 2011 Jul;40(4):468-71.
OBJECTIVE
To study the effects of inorganic arsenic on mRNA expression of transcription factor Nrf2 and Nrf2-regulated downstream anti-oxidant enzymes, NADPH: quinine oxidoreductase 1 (NQO1) and heme oxygenase 1(HO-1) in Chang hepatocytes.
METHODS
Chang hepatocytes were treated with 5, 10, 25 and 50 micromol/L of sodium arsenite (NaAsO2) for 6h, and RT-PCR were then performed to detect the mRNA expression of Nrf2, NQO1 and HO-1.
RESULTS
When exposed to 5, 10, 25 and 50 micromol/L of NaAsO2, Nrf2 mRNA were (100.74 +/- 3.70)%, (105.96 +/- 1.75)%, (101.76 +/- 1.01)% and (101.81 +/- 6.33)% of control,showing no statistic significance (P > 0.05). NQO1 mRNA expression were significantly increased (P < 0.05), and the levels of NQO1 mRNA were (106.52 +/- 3.11)%, (113.27 +/- 2.84)%, (111.96 +/- 6.96)% and (107.33 +/- 2.76)% of control, respectively. Moreover, HO-1 mRNA expression were also significantly induced by sodium arsenite exposure, and a definite dose-effect relationship was confirmed (P < 0.01).
CONCLUSION
No elevation of Nrf2 transcription was found, while inorganic arsenic could increase the Nrf2-regulated NQO1 and HO-1 mRNA expression in human hepatocytes.
目的
研究无机砷对Chang肝细胞中转录因子Nrf2及其调控的下游抗氧化酶NADPH：醌氧化还原酶1（NQO1）和血红素加氧酶1（HO-1）mRNA表达的影响。
方法
用5、10、25和50 μmol/L的亚砷酸钠（NaAsO₂）处理Chang肝细胞6小时，然后进行RT-PCR检测Nrf2、NQO1和HO-1的mRNA表达。
结果
当暴露于5、10、25和50 μmol/L的NaAsO₂时，Nrf2 mRNA分别为对照组的（100.74±3.70）%、（105.96±1.75）%、（101.76±1.01）%和（101.81±6.33）%，差异无统计学意义（P>0.05）。NQO1 mRNA表达显著增加（P<0.05），其水平分别为对照组的（106.52±3.11）%、（113.27±2.84）%、（111.96±6.96）%和（107.33±2.76）%。此外，HO-1 mRNA表达也被亚砷酸钠暴露显著诱导，并证实有明确的剂量-效应关系（P<0.01）。
结论
未发现Nrf2转录升高，而无机砷可增加人肝细胞中Nrf2调控的NQO1和HO-1 mRNA表达。
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