Clausen Martijn, Nakagomi Takayuki, Nakano-Doi Akiko, Saino Orie, Takata Masashi, Taguchi Akihiko, Luiten Paul, Matsuyama Tomohiro
Institute for Advanced Medical Sciences, Hyogo College of Medicine, Nishinomiya, Hyogo, Japan.
Neuroreport. 2011 Nov 16;22(16):789-94. doi: 10.1097/WNR.0b013e32834acb54.
Adult brain-derived neural stem cells have acquired a lot of interest as an endurable neuronal cell source that can be used for central nervous system repair in a wide range of neurological disorders such as ischemic stroke. Recently, we identified injury-induced neural stem/progenitor cells in the poststroke murine cerebral cortex. In this study, we show that, after differentiation in vitro, injury-induced neural stem/progenitor cells express pyramidal cell markers Emx1 and CaMKIIα, as well as mature neuron markers MAP2 and Tuj1. 5-bromo-2-deoxyuridinine-positive neurons in the peristroke cortex also express such pyramidal markers. The presence of newly regenerated pyramidal neurons in the poststroke brain might provide a noninvasive therapeutic strategy for stroke treatment with functional recovery.
成体脑源性神经干细胞作为一种持久的神经元细胞来源，已引起广泛关注，可用于修复多种神经系统疾病（如缺血性中风）中的中枢神经系统。最近，我们在中风后小鼠大脑皮层中鉴定出损伤诱导的神经干/祖细胞。在本研究中，我们发现，体外分化后，损伤诱导的神经干/祖细胞表达锥体细胞标志物Emx1和CaMKIIα，以及成熟神经元标志物MAP2和Tuj1。中风周围皮层中5-溴-2-脱氧尿苷阳性神经元也表达此类锥体标志物。中风后大脑中新再生的锥体神经元的存在可能为中风治疗及功能恢复提供一种非侵入性治疗策略。
