São Francisco University, São Francisco de Assis Av, 218, Bragança Paulista, SP 12916-900, Brazil.
Molecules. 2011 Aug 23;16(8):7171-82. doi: 10.3390/molecules16087171.
Response surface methodology (RSM) based on a five-level, three-variable central composite design (CCD) was employed for modeling and optimizing the conversion yield of the enzymatic acylation of hesperidin with decanoic acid using immobilized Candida antarctica lipase B (CALB) in a two-phase system containing [bmim]BF(4). The three variables studied (molar ratio of hesperidin to decanoic acid, [bmim]BF(4)/acetone ratio and lipase concentration) significantly affected the conversion yield of acylated hesperidin derivative. Verification experiments confirmed the validity of the predicted model. The lipase showed higher conversion degree in a two-phase system using [bmim]BF(4) and acetone compared to that in pure acetone. Under the optimal reaction conditions carried out in a single-step biocatalytic process when the water content was kept lower than 200 ppm, the maximum acylation yield was 53.6%.
采用基于五水平三变量中心组合设计（CCD）的响应面法（RSM），对固定化南极假丝酵母脂肪酶 B（CALB）在两相体系中酶促酰化橙皮苷与癸酸的转化率进行建模和优化，该两相体系含有[bmim]BF(4)。研究的三个变量（橙皮苷与癸酸的摩尔比、[bmim]BF(4)/丙酮的比例和脂肪酶浓度）显著影响了酰化橙皮苷衍生物的转化率。验证实验证实了预测模型的有效性。与纯丙酮相比，脂肪酶在两相体系中使用[bmim]BF(4)和丙酮时的转化率更高。在单步生物催化过程中，当水含量保持在 200ppm 以下时，在优化反应条件下，最大酰化产率为 53.6%。
