ERC for Advanced Bioseparation Technology, Inha University, Incheon 402-751, Korea.
Bioprocess Biosyst Eng. 2010 Jan;33(1):63-70. doi: 10.1007/s00449-009-0363-4. Epub 2009 Aug 13.
Lipase-catalyzed esterification of glucose with fatty acids in ionic liquids (ILs) mixture was investigated by using supersaturated glucose solution. The effect of ILs mixture ratio, substrate ratio, lipase content, and temperature on the activity and stability of lipase was also studied. The highest yield of sugar ester was obtained in a mixture of 1-butyl-3-methylimidazolium trifluoromethanesulfonate ([Bmim][TfO]) and 1-methyl-3-octylimidazolium bis[(trifluoromethyl)-sulfonyl]amide ([Omim][Tf(2)N]) with a volume ratio of 9:1, while Novozym 435 (Candida antarctica type B lipase immobilized on acrylic resin) showed the optimal stability and activity in a mixture of [Bmim][TfO] and [Omim][Tf(2)N] with a 1:1 volume ratio. Reuse of lipase and ILs was successfully carried out at the optimized reaction conditions. After 5 times reuse of Novozym 435 and ILs, 78% of initial activity was remained.
在超饱和葡萄糖溶液中，研究了脂肪酶在离子液体（ILs）混合物中催化葡萄糖与脂肪酸的酯化反应。还研究了 ILs 混合物比例、底物比例、脂肪酶含量和温度对脂肪酶活性和稳定性的影响。在 1-丁基-3-甲基咪唑三氟甲磺酸盐（[Bmim][TfO]）和 1-甲基-3-辛基咪唑双（三氟甲磺酰基）酰胺（[Omim][Tf(2)N]）体积比为 9:1 的混合物中，获得了最高的糖酯产率，而 Novozym 435（固定在丙烯酸树脂上的南极假丝酵母 B 型脂肪酶）在 [Bmim][TfO]和 [Omim][Tf(2)N]体积比为 1:1 的混合物中表现出最佳的稳定性和活性。在优化的反应条件下，成功地进行了脂肪酶和 ILs 的重复使用。Novozym 435 和 ILs 重复使用 5 次后，初始活性保留了 78%。
