• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

CRIPTO-1 在皮肤黑色素瘤中的表达和功能作用。

Expression and functional role of CRIPTO-1 in cutaneous melanoma.

机构信息

Cell Biology and Biotherapy Unit, Research Department, INT-Fondazione Pascale, Naples 80131, Italy.

出版信息

Br J Cancer. 2011 Sep 27;105(7):1030-8. doi: 10.1038/bjc.2011.324. Epub 2011 Aug 23.

DOI:10.1038/bjc.2011.324
PMID:21863025
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3185940/
Abstract

BACKGROUND

CRIPTO-1 (CR-1) is involved in the pathogenesis and progression of human carcinoma of different histological origin. In this study we addressed the expression and the functional role of CR-1 in cutaneous melanoma.

METHODS

Expression of CR-1 protein in melanomas and melanoma cell lines was assessed by immunohistochemistry, western blotting and/or flow cytometry. Levels of mRNA were evaluated by real-time PCR. Invasion assays were performed in Matrigel-coated modified Boyden chambers.

RESULTS

Expression of CR-1 protein and/or mRNA was found in 16 out of 37 primary human cutaneous melanomas and in 12 out of 21 melanoma cell lines. Recombinant CR-1 protein activated in melanoma cells c-Src and, at lesser extent, Smad signalling. In addition, CR-1 significantly increased the invasive ability of melanoma cells that was prevented by treatment with either the ALK4 inhibitor SB-431542 or the c-Src inhibitor saracatinib (AZD0530). Anti-CR-1 siRNAs produced a significant inhibition of the growth and the invasive ability of melanoma cells. Finally, a close correlation was found in melanoma cells between the levels of expression of CR-1 and the effects of saracatinib on cell growth.

CONCLUSION

These data indicate that a significant fraction of cutaneous melanoma expresses CR-1 and that this growth factor is involved in the invasion and proliferation of melanoma cells.

摘要

背景

CRIPTO-1（CR-1）参与了不同组织来源的人类癌的发病机制和进展。在这项研究中，我们研究了 CR-1 在皮肤黑素瘤中的表达和功能作用。

方法

通过免疫组织化学、western blot 和/或流式细胞术评估黑素瘤和黑素瘤细胞系中 CR-1 蛋白的表达。通过实时 PCR 评估 mRNA 水平。在 Matrigel 包被的改良 Boyden 室中进行侵袭实验。

结果

在 37 例原发性人类皮肤黑素瘤中的 16 例和 21 例黑素瘤细胞系中的 12 例中发现了 CR-1 蛋白和/或 mRNA 的表达。重组 CR-1 蛋白在黑素瘤细胞中激活 c-Src，并且在较小程度上激活 Smad 信号。此外，CR-1 显著增加了黑素瘤细胞的侵袭能力，而 ALK4 抑制剂 SB-431542 或 c-Src 抑制剂 saracatinib（AZD0530）的处理则阻止了这种侵袭能力的增加。抗 CR-1 siRNAs 显著抑制了黑素瘤细胞的生长和侵袭能力。最后，在黑素瘤细胞中发现 CR-1 的表达水平与 saracatinib 对细胞生长的影响之间存在密切相关性。

结论

这些数据表明，相当一部分皮肤黑素瘤表达 CR-1，并且这种生长因子参与了黑素瘤细胞的侵袭和增殖。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e29c/3185940/9e09e8223c23/bjc2011324f7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e29c/3185940/98ef217184a7/bjc2011324f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e29c/3185940/5c99d0dfc091/bjc2011324f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e29c/3185940/bfc545cc356f/bjc2011324f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e29c/3185940/251c4fa34133/bjc2011324f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e29c/3185940/b684472805a9/bjc2011324f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e29c/3185940/51cff6c54ce3/bjc2011324f6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e29c/3185940/9e09e8223c23/bjc2011324f7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e29c/3185940/98ef217184a7/bjc2011324f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e29c/3185940/5c99d0dfc091/bjc2011324f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e29c/3185940/bfc545cc356f/bjc2011324f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e29c/3185940/251c4fa34133/bjc2011324f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e29c/3185940/b684472805a9/bjc2011324f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e29c/3185940/51cff6c54ce3/bjc2011324f6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e29c/3185940/9e09e8223c23/bjc2011324f7.jpg

相似文献

1
Expression and functional role of CRIPTO-1 in cutaneous melanoma.CRIPTO-1 在皮肤黑色素瘤中的表达和功能作用。
Br J Cancer. 2011 Sep 27;105(7):1030-8. doi: 10.1038/bjc.2011.324. Epub 2011 Aug 23.
2
Knockdown of Cripto-1 inhibits the proliferation, migration, invasion, and angiogenesis in prostate carcinoma cells.敲低Cripto-1可抑制前列腺癌细胞的增殖、迁移、侵袭和血管生成。
J Biosci. 2017 Sep;42(3):405-416. doi: 10.1007/s12038-017-9700-y.
3
Gene expression profiles of human melanoma cells with different invasive potential reveal TSPAN8 as a novel mediator of invasion.不同侵袭潜能的人黑色素瘤细胞的基因表达谱揭示 TSPAN8 是一种新型侵袭介质。
Br J Cancer. 2011 Jan 4;104(1):155-65. doi: 10.1038/sj.bjc.6605994. Epub 2010 Nov 16.
4
Cripto-1 promotes epithelial-mesenchymal transition in prostate cancer via Wnt/β-catenin signaling.Cripto-1通过Wnt/β-连环蛋白信号通路促进前列腺癌上皮-间质转化。
Oncol Rep. 2017 Mar;37(3):1521-1528. doi: 10.3892/or.2017.5378. Epub 2017 Jan 17.
5
HOXC11-SRC-1 regulation of S100beta in cutaneous melanoma: new targets for the kinase inhibitor dasatinib.HOXC11-SRC-1 调控皮肤黑色素瘤中的 S100β：激酶抑制剂 dasatinib 的新靶点。
Br J Cancer. 2011 Jun 28;105(1):118-23. doi: 10.1038/bjc.2011.193. Epub 2011 Jun 7.
6
Hyaluronan-CD44 interaction promotes c-Src-mediated twist signaling, microRNA-10b expression, and RhoA/RhoC up-regulation, leading to Rho-kinase-associated cytoskeleton activation and breast tumor cell invasion.透明质酸-CD44 相互作用促进 c-Src 介导的 twist 信号转导、microRNA-10b 的表达以及 RhoA/RhoC 的上调，导致 Rho 激酶相关细胞骨架激活和乳腺癌细胞侵袭。
J Biol Chem. 2010 Nov 19;285(47):36721-35. doi: 10.1074/jbc.M110.162305. Epub 2010 Sep 15.
7
Cripto-1 acts as a functional marker of cancer stem-like cells and predicts prognosis of the patients in esophageal squamous cell carcinoma.Cripto-1作为癌症干细胞样细胞的功能标志物，可预测食管鳞状细胞癌患者的预后。
Mol Cancer. 2017 Apr 21;16(1):81. doi: 10.1186/s12943-017-0650-7.
8
The multifaceted role of the embryonic gene Cripto-1 in cancer, stem cells and epithelial-mesenchymal transition.胚胎基因Cripto-1在癌症、干细胞及上皮-间质转化中的多方面作用
Semin Cancer Biol. 2014 Dec;29:51-8. doi: 10.1016/j.semcancer.2014.08.003. Epub 2014 Aug 19.
9
The role of Cripto-1 in the tumorigenesis and progression of oral squamous cell carcinoma.Cripto-1 在口腔鳞状细胞癌发生和发展中的作用。
Oral Oncol. 2011 Nov;47(11):1023-31. doi: 10.1016/j.oraloncology.2011.07.019. Epub 2011 Aug 6.
10
A Nodal- and ALK4-independent signaling pathway activated by Cripto-1 through Glypican-1 and c-Src.一种由Cripto-1通过Glypican-1和c-Src激活的不依赖Nodal和ALK4的信号通路。
Cancer Res. 2003 Mar 15;63(6):1192-7.

引用本文的文献

1
Cripto-1 and Cripto-3 differential expression pattern in human placenta, umbilical cord and fetal tissues suggests new functions for these proteins.Cripto-1和Cripto-3在人胎盘、脐带和胎儿组织中的差异表达模式提示了这些蛋白质的新功能。
Sci Rep. 2025 Aug 20;15(1):30497. doi: 10.1038/s41598-025-14201-x.
2
Human Cripto-1 and Cripto-3 Protein Expression in Normal and Malignant Settings That Conflicts with Established Conventions.人类Cripto-1和Cripto-3蛋白在正常和恶性环境中的表达与既定规范相冲突。
Cancers (Basel). 2024 Oct 23;16(21):3577. doi: 10.3390/cancers16213577.
3
Expression and prognostic value of Cripto-1 in early non-small cell lung cancer.

本文引用的文献

1
Nodal as a biomarker for melanoma progression and a new therapeutic target for clinical intervention.Nodal作为黑色素瘤进展的生物标志物及临床干预的新治疗靶点。
Expert Rev Dermatol. 2009;4(1):67-78. doi: 10.1586/17469872.4.1.67.
2
Src activation in melanoma and Src inhibitors as therapeutic agents in melanoma.黑色素瘤中的Src激活以及Src抑制剂作为黑色素瘤治疗药物的研究
Melanoma Res. 2009 Jun;19(3):167-75. doi: 10.1097/CMR.0b013e328304974c.
3
Src, PKCalpha, and PKCdelta are required for alphavbeta3 integrin-mediated metastatic melanoma invasion.
Cripto-1 在早期非小细胞肺癌中的表达及预后价值。
Clin Respir J. 2023 Dec;17(12):1203-1208. doi: 10.1111/crj.13680. Epub 2023 Aug 1.
4
CRIPTO Is a Marker of Chemotherapy-Induced Stem Cell Expansion in Non-Small Cell Lung Cancer.CRIPTO是化疗诱导的非小细胞肺癌干细胞扩增的标志物。
Front Oncol. 2022 Jun 2;12:830873. doi: 10.3389/fonc.2022.830873. eCollection 2022.
5
The EGFR Signaling Modulates in Mesenchymal Stem Cells the Expression of miRNAs Involved in the Interaction with Breast Cancer Cells.表皮生长因子受体（EGFR）信号传导调节间充质干细胞中参与与乳腺癌细胞相互作用的微小RNA（miRNA）的表达。
Cancers (Basel). 2022 Apr 6;14(7):1851. doi: 10.3390/cancers14071851.
6
Normal Skin Cells Increase Aggressiveness of Cutaneous Melanoma by Promoting Epithelial-to-Mesenchymal Transition via Nodal and Wnt Activity.正常皮肤细胞通过促进上皮间质转化增加皮肤黑色素瘤的侵袭性，其途径为激活 nodal 和 Wnt 信号通路。
Int J Mol Sci. 2021 Oct 29;22(21):11719. doi: 10.3390/ijms222111719.
7
Whence CRIPTO: The Reemergence of an Oncofetal Factor in 'Wounds' That Fail to Heal.CRIPTO 源自何方：在未能愈合的“伤口”中重新出现的一种癌胚因子。
Int J Mol Sci. 2021 Sep 21;22(18):10164. doi: 10.3390/ijms221810164.
8
Cripto-1 as a Key Factor in Tumor Progression, Epithelial to Mesenchymal Transition and Cancer Stem Cells.Cripto-1 作为肿瘤进展、上皮间质转化和癌症干细胞的关键因素。
Int J Mol Sci. 2021 Aug 27;22(17):9280. doi: 10.3390/ijms22179280.
9
Cripto-1 as a Potential Target of Cancer Stem Cells for Immunotherapy.Cripto-1作为癌症干细胞免疫治疗的潜在靶点
Cancers (Basel). 2021 May 20;13(10):2491. doi: 10.3390/cancers13102491.
10
A Multidisciplinary Review of the Roles of Cripto in the Scientific Literature Through a Bibliometric Analysis of its Biological Roles.通过对CRIPTO生物学作用的文献计量分析对科学文献中CRIPTO的作用进行多学科综述。
Cancers (Basel). 2020 Jun 5;12(6):1480. doi: 10.3390/cancers12061480.
Src、PKCalpha 和 PKCdelta 对于 alpha v beta 3 整合素介导的转移性黑色素瘤侵袭是必需的。
Cell Commun Signal. 2009 Apr 28;7:10. doi: 10.1186/1478-811X-7-10.
4
Inhibition of Src family kinases with dasatinib blocks migration and invasion of human melanoma cells.达沙替尼抑制Src家族激酶可阻断人黑色素瘤细胞的迁移和侵袭。
Mol Cancer Res. 2008 Nov;6(11):1766-74. doi: 10.1158/1541-7786.MCR-08-0169.
5
Cripto localizes Nodal at the limiting membrane of early endosomes.Cripto将Nodal定位在早期内体的限制膜上。
Sci Signal. 2008 Nov 11;1(45):ra13. doi: 10.1126/scisignal.1165027.
6
Preclinical evaluation of dasatinib, a potent Src kinase inhibitor, in melanoma cell lines.强效Src激酶抑制剂达沙替尼在黑色素瘤细胞系中的临床前评估。
J Transl Med. 2008 Sep 29;6:53. doi: 10.1186/1479-5876-6-53.
7
Cripto recruits Furin and PACE4 and controls Nodal trafficking during proteolytic maturation.Cripto招募弗林蛋白酶和PACE4，并在蛋白水解成熟过程中控制Nodal的运输。
EMBO J. 2008 Oct 8;27(19):2580-91. doi: 10.1038/emboj.2008.174. Epub 2008 Sep 4.
8
Potential for cripto-1 in defining stem cell-like characteristics in human malignant melanoma.Cripto-1在定义人类恶性黑色素瘤干细胞样特征方面的潜力。
Cell Cycle. 2008 Jul 1;7(13):1931-5. doi: 10.4161/cc.7.13.6236. Epub 2008 May 5.
9
Human embryonic stem cell microenvironment suppresses the tumorigenic phenotype of aggressive cancer cells.人类胚胎干细胞微环境抑制侵袭性癌细胞的致瘤表型。
Proc Natl Acad Sci U S A. 2008 Mar 18;105(11):4329-34. doi: 10.1073/pnas.0800467105. Epub 2008 Mar 11.
10
Cripto-1 expression in uveal melanoma: an immunohistochemical study.葡萄膜黑色素瘤中Cripto-1的表达：一项免疫组织化学研究。
Exp Eye Res. 2007 Jun;84(6):1060-6. doi: 10.1016/j.exer.2007.01.019. Epub 2007 Feb 7.