Hafez Nesreen H, Tahoun Neveen S
The Department of Pathology, Cytopathology Unit, National Cancer Institute (NCI), Cairo University.
J Egypt Natl Canc Inst. 2010 Dec;22(4):217-25.
Evaluation of the reliability of immunocytochemical staining for estrogen and progesterone receptor status on previously papanicolaou-stained fine needle aspiration smears of breast carcinoma cases.
This is a retrospective study conducted on destained smears of fine needle aspirates (FNA) obtained from 90 breast carcinoma cases. These cases underwent subsequent tumor resection and immunohistochemical detection of estrogen and progesterone receptors allowing a comparison between the immunocytochemical and immunohistochemical results (Gold Standard). Hypocelluar slides were excluded from the current study. Only the nuclear staining was considered specific. The results were scored on the basis of the percentage of the positive nuclei among the total epithelial malignant cells after examination of the entire slide. Smears were interpreted as positive if 10% of the examined cells demonstrated nuclear staining. These results were then compared with the immunohistochemical results.
For estrogen receptor immunocytochemistry, the overall cyto-histologic accuracy was 91.1% (82÷90) while the discordance rate was 8.9% (8÷90). The diagnostic sensitivity, specificity, positive predictive value, and negative predictive value were 93%, 84.2%, 95.7%, and 76.2% respectively. For progesterone receptor immunocytochemistry, the overall cyto-histologic accuracy was 88.9% (80÷90) while the discordance rate was 11.1% (10÷90). The diagnostic sensitivity, specificity, positive predictive value, and negative predictive value were 87.1%, 95%, 98.4%, and 67.9% respectively.
Immunocytochemistry is considered as an efficient tool in evaluating estrogen and progesterone receptor status in breast carcinoma. The application of estrogen and progesterone receptor immunocytochemistry on previously Papanicolaou-stained slides provides an overall accuracy of 91.1% for estrogen receptor and 88.9% for progesterone receptor when compared with the immunohistochemical results.
Estrogen receptor- Progesterone receptor- Breast carcinoma- FNA- Immunocytochemistry.
评估在先前经巴氏染色的乳腺癌细针穿刺涂片上进行雌激素和孕激素受体状态免疫细胞化学染色的可靠性。
这是一项对90例乳腺癌患者的细针穿刺（FNA）脱色涂片进行的回顾性研究。这些病例随后接受了肿瘤切除及雌激素和孕激素受体的免疫组织化学检测，从而能够比较免疫细胞化学和免疫组织化学结果（金标准）。细胞数量少的玻片被排除在本研究之外。仅细胞核染色被视为特异性染色。在检查整个玻片后，根据阳性细胞核在全部上皮恶性细胞中的百分比对结果进行评分。如果10%的被检查细胞显示细胞核染色，则涂片被解释为阳性。然后将这些结果与免疫组织化学结果进行比较。
对于雌激素受体免疫细胞化学，总体细胞 - 组织学准确性为91.1%（82÷90），不一致率为8.9%（8÷90）。诊断敏感性、特异性、阳性预测值和阴性预测值分别为93%、84.2%、95.7%和76.2%。对于孕激素受体免疫细胞化学，总体细胞 - 组织学准确性为88.9%（80÷90），不一致率为11.1%（10÷90）。诊断敏感性、特异性、阳性预测值和阴性预测值分别为87.1%、95%、98.4%和67.9%。
免疫细胞化学被认为是评估乳腺癌中雌激素和孕激素受体状态的有效工具。与免疫组织化学结果相比，在先前经巴氏染色的玻片上应用雌激素和孕激素受体免疫细胞化学，雌激素受体的总体准确性为91.1%，孕激素受体为88.9%。
雌激素受体 - 孕激素受体 - 乳腺癌 - FNA - 免疫细胞化学
