Klipp Edda
Theoretical Biophysics, Humboldt-Universität zu Berlin, Berlin, Germany.
Methods Mol Biol. 2011;759:323-43. doi: 10.1007/978-1-61779-173-4_19.
Cellular networks and processes can be mathematically described and analyzed in various ways. Here, the case example of a MAP kinase (MAPK) cascade is used to detail steps in the formulation of a system of ordinary differential equations governing the temporal behavior of a signal transduction pathway after stimulation. Different analysis methods for the model are explained and demonstrated, such as stoichiometric analysis, sensitivity analysis, or studying the effect of deletions and protein overexpression. Finally, a perspective on standards concerning modeling in systems biology is given.
细胞网络和过程可以通过多种方式进行数学描述和分析。在此，以丝裂原活化蛋白激酶（MAPK）级联反应为例，详细阐述构建常微分方程组的步骤，该方程组用于描述刺激后信号转导通路的时间行为。文中解释并演示了该模型的不同分析方法，如化学计量分析、敏感性分析，或研究基因缺失和蛋白质过表达的影响。最后，给出了关于系统生物学建模标准的观点。
