基于内转录间隔区-RFLP、ISSR和内含子标记的绿僵菌变种金龟子绿僵菌（肉座菌目：麦角菌科）的多态性

Polymorphism in Metarhizium anisopliae var. anisopliae (Hypocreales: Clavicipitaceae) based on internal transcribed spacer-RFLP, ISSR and intron markers.

作者信息

Tiago P V, Carneiro-Leão M P, Lima M L A, Oliveira N T, Luna-Alves Lima E A

机构信息

Departamento de Micologia, Universidade Federal de Pernambuco, Recife, PE, Brasil.

出版信息

Genet Mol Res. 2011;10(3):1565-75. doi: 10.4238/vol10-3gmr1069.

DOI:10.4238/vol10-3gmr1069

PMID:21863552

Abstract

Isolates of entomopathogenic fungus Metarhizium anisopliae var. anisopliae were characterized using internal transcribed spacer-RFLP, ISSR and intron splice site primers. Thirty-seven isolates were studied, most of which were obtained from the sugar cane pest, Mahanarva fimbriolata (Hemiptera: Cercopidae) from Tangará da Serra, Southwest Mato Grosso State, Brazil. Internal transcribed spacer-RFLP did not differentiate the isolates of M. anisopliae var. anisopliae, while ISSR and intron primers identified three distinct groups. Variability among these groups was 96% for (GTG)(5) and 100% for the other primers. We found considerable genetic variability, even among isolates from the same geographical origin and host.

摘要

利用内转录间隔区限制性片段长度多态性（internal transcribed spacer-RFLP）、简单序列重复区间（ISSR）和内含子剪接位点引物对昆虫病原真菌绿僵菌金龟子变种（Metarhizium anisopliae var. anisopliae）的分离株进行了特征分析。研究了37个分离株，其中大部分分离株取自巴西马托格罗索州西南部坦加拉达塞拉的甘蔗害虫细纹玛哈纳若叶蝉（Mahanarva fimbriolata，半翅目：沫蝉科）。内转录间隔区限制性片段长度多态性未能区分绿僵菌金龟子变种的分离株，而简单序列重复区间和内含子引物鉴定出三个不同的组。这些组之间的变异率对于（GTG）(5) 为96%，对于其他引物为100%。我们发现，即使在来自相同地理区域和寄主的分离株之间也存在相当大的遗传变异。