Enkerli Jürg, Ghormade Vandana, Oulevey Catherine, Widmer Franco
Agroscope Reckenholz-Tänikon Research Station ART, Reckenholzstrasse 191, 8046 Zürich, Switzerland.
J Invertebr Pathol. 2009 Oct;102(2):185-8. doi: 10.1016/j.jip.2009.08.006. Epub 2009 Aug 12.
A new genotyping tool has been developed and evaluated for Metarhizium anisopliae var. anisopliae. The tool is based on Restriction Fragment Length Polymorphism (RFLP) analysis of three chitinase genes that are functionally linked to insect-pathogenicity of this fungus. It allowed for discrimination of 14 genotypes among 22 M. anisopliae var. anisopliae strains of a world wide collection. Analyses revealed that the approach may also be applicable to other Metarhizium varieties. The new tool will be useful for genetic characterization of M. anisopliae var. anisopliae strains, and it is applicable for laboratories with limited access to molecular diagnostic equipment.
一种针对绿僵菌变种金龟子绿僵菌的新型基因分型工具已被开发并进行了评估。该工具基于对三个几丁质酶基因的限制性片段长度多态性（RFLP）分析，这三个基因在功能上与该真菌的昆虫致病性相关。它能够区分来自全球范围内收集的22株金龟子绿僵菌变种金龟子绿僵菌中的14种基因型。分析表明，该方法也可能适用于其他绿僵菌变种。这种新工具将有助于金龟子绿僵菌变种金龟子绿僵菌菌株的遗传特征分析，并且适用于难以获得分子诊断设备的实验室。
