方法：一种用于谷物植物分子分析的高质量DNA提取改良方法。
Methodology: a modified method for high-quality DNA extraction for molecular analysis in cereal plants.
作者信息
Pervaiz Z H, Turi N A, Khaliq I, Rabbani M A, Malik S A
机构信息
Department of Biochemistry, Quaid-e-Azam University, Islamabad, Pakistan.
出版信息
Genet Mol Res. 2011;10(3):1669-73. doi: 10.4238/vol10-3gmr1346.
Cereal crops that have rigid non-cellulose components in the cell wall tissues of leaves and high starch and protein content in grains face limitations in DNA extraction. Advanced molecular genetic techniques such as mapping and marker-assisted selection programs require pure and quick DNA extraction. In this study, we developed methods for isolating high-quality genomic DNA from leaves and seeds of major cereal crops with minor modifications. DNA yields ranged from 300 to 1800 ng for 0.01 g seed or leaf tissue.
在叶片细胞壁组织中含有坚硬非纤维素成分且种子中淀粉和蛋白质含量高的谷类作物，在DNA提取方面面临限制。诸如作图和标记辅助选择计划等先进的分子遗传技术需要纯净且快速的DNA提取。在本研究中，我们对主要谷类作物的叶片和种子进行了微小改良，开发出了分离高质量基因组DNA的方法。对于0.01克种子或叶片组织，DNA产量在300至1800纳克之间。
Zotero 插件