Huang Bao-ying, Wang Xiu-ping, Tang Wen-jie, Wang Wen-ling, Ruan Li
National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 100052, China.
Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi. 2011 Apr;25(2):92-5.
To evaluate the seasonal influenza spilt vaccine's immunogenicity and the 50% effective dose (ED50a) of hemagglutin (HA) that can make 50% of the mice hemagglutination inhibition antibody (HI) titers to 40.
The 2008-2009 seasonal influenza spilt vaccine's two components, with HA from H1N1 and H3N2 influenza virus respectively, were used as a model. Mice were immunized once or twice with different doses, and the HI antibody titers were tested to determine the immunization procedure and to evaluate the immugenicity of seasonal influenza spilt vaccine in mice; Consequently, HI antibody response kinetics of the two components were observed to determine the time point when the HI antibody titer reached the peak point; Finally, mice were immunized with different doses of HA to evaluate the ED50a that can make 50% of mice HI titers reach 40.
Immunization procedures study showed that one-dose of seasonal influenza vaccine induced the HI antibody titers ranged from 10 to 120, while two-dose of influenza vaccine improved the HI antibody titer 10-100 times as compared with one dose; antibody kinetics study suggested that the time point of HI antibody produced to peak is 28-35 days post one dose immunization; and the ED50a detection results indicated that one dose of 1.5 microg HA could make 50% of the mice HI antibody titer reach 40.
Seasonal influenza spilt vaccine is very immunogenic in mouse; the time point of HI antibody produced to peak is 28-35 days post one dose immunization; and the ED50a of HA is 1.5 microg, which can make 50% of the mice HI titer reach 40. The experimental results provided foundation for the establishment of influenza vaccine evaluation system based on seasonal influenza vaccine.
评估季节性流感裂解疫苗的免疫原性以及能使50%小鼠血凝抑制抗体（HI）效价达到40的血凝素（HA）的50%有效剂量（ED50a）。
以2008 - 2009年季节性流感裂解疫苗的两个组分（分别含有来自H1N1和H3N2流感病毒的HA）为模型。用不同剂量对小鼠进行一次或两次免疫，检测HI抗体效价以确定免疫程序并评估季节性流感裂解疫苗在小鼠中的免疫原性；随后，观察两个组分的HI抗体反应动力学以确定HI抗体效价达到峰值的时间点；最后，用不同剂量的HA对小鼠进行免疫以评估能使50%小鼠HI效价达到40的ED50a。
免疫程序研究表明，一剂季节性流感疫苗诱导的HI抗体效价范围为10至120，而两剂流感疫苗使HI抗体效价比一剂提高了10 - 100倍；抗体动力学研究表明，一剂免疫后HI抗体产生至峰值的时间点为接种后28 - 35天；ED50a检测结果表明，1.5微克HA一剂可使50%小鼠的HI抗体效价达到40。
季节性流感裂解疫苗在小鼠中具有很强的免疫原性；一剂免疫后HI抗体产生至峰值的时间点为接种后28 - 35天；HA的ED50a为1.5微克，可使50%小鼠的HI效价达到40。实验结果为基于季节性流感疫苗建立流感疫苗评价体系提供了依据。
