Gansu College of Traditional Chinese Medicine, Lanzhou, Gansu, China.
Immunol Invest. 2012;41(4):356-66. doi: 10.3109/08820139.2011.606861. Epub 2011 Aug 24.
It is of fundamental importance to use an appropriate adjuvant to generate a potent immune response for immunotherapy. In this study, we had a comparative investigation on the effectiveness of two adjuvant formulations, liposome-polycation-DNA (LPD) and monophosphoryl lipid A(MPL) in combination with a truncated peptide of bFGF(tbFGF) as antigen. LPD/tbFGF induced continuously increasing antibodies expression during the whole immunization period. In contrast, the level of antibodies was variable in MPL/tbFGF-immunized mice, MPL/tbFGF elicited potent antibodies response in the early-phase of immunization (during the first 3 immunizations), but the later immunizations did not produce a significant increase in the level of antibodies. Evaluation of IFN-γ and IL-4 responses revealed that both LPD/tbFGF and MPL/tbFGF demonstrated generation of higher level of IFN-γ, whereas no significant increase in IL-4 levels was detected in the two groups. In addition, histological analysis exhibited obvious germinal centers in the spleen tissues of LPD/tbFGF mice. The data suggested that LPD would be a promising long-effective adjuvant due to its potent and persistent immunostimulation and MPL could play an appropriate role in short-acting immunization.
使用适当的佐剂来产生有效的免疫应答对于免疫疗法至关重要。在这项研究中，我们比较了两种佐剂制剂——脂质体-聚阳离子-DNA（LPD）和单磷酰脂质 A（MPL）与 bFGF 的截短肽（tbFGF）作为抗原的效果。LPD/tbFGF 在整个免疫期间诱导持续增加的抗体表达。相比之下，MPL/tbFGF 免疫的小鼠的抗体水平是可变的，MPL/tbFGF 在免疫的早期（前 3 次免疫期间）产生了强烈的抗体反应，但随后的免疫没有显著增加抗体水平。IFN-γ 和 IL-4 反应的评估表明，LPD/tbFGF 和 MPL/tbFGF 均产生了更高水平的 IFN-γ，而两组的 IL-4 水平均没有显著增加。此外，组织学分析显示 LPD/tbFGF 小鼠的脾脏组织中存在明显的生发中心。这些数据表明，由于其强大而持久的免疫刺激作用，LPD 将成为一种有前途的长效佐剂，而 MPL 可以在短期免疫中发挥适当的作用。
