一种新型病毒编码的核质穿梭蛋白：单纯疱疹病毒 1 的 UL3 蛋白。
A novel virus-encoded nucleocytoplasmic shuttling protein: the UL3 protein of herpes simplex virus type 1.
机构信息
Molecular Virology and Viral Immunology Research Group, State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Science, 44 Xiaohongshan, Wuchang, Wuhan 430071, China.
出版信息
J Virol Methods. 2011 Nov;177(2):206-10. doi: 10.1016/j.jviromet.2011.08.004. Epub 2011 Aug 11.
Herpes simplex virus type 1 (HSV-1) UL3 protein is a nuclear protein. In this study, the molecular mechanism of the subcellular localization of UL3 was characterized by fluorescence microscopy in living cells. A nuclear localization signal (NLS) and a nuclear export signal (NES) were also identified. UL3 was demonstrated to target to the cytoplasm through the NES via chromosomal region maintenance 1 (CRM-1) dependent pathway, and to the nucleus through RanGTP-dependent mechanism. Heterokaryon assays confirmed that UL3 was capable of shuttling between the nucleus and the cytoplasm. These results demonstrate that the UL3 protein is a novel HSV-1 encoded nucleocytoplasmic shuttling protein.
单纯疱疹病毒 1 型 (HSV-1) UL3 蛋白是一种核蛋白。在这项研究中，通过活细胞中的荧光显微镜研究了 UL3 的亚细胞定位的分子机制。还鉴定了核定位信号 (NLS) 和核输出信号 (NES)。通过染色体区域维持 1 (CRM-1) 依赖性途径，UL3 被证明通过 NES 靶向细胞质，并且通过 RanGTP 依赖性机制靶向细胞核。异核体测定证实 UL3 能够在核和细胞质之间穿梭。这些结果表明 UL3 蛋白是一种新型 HSV-1 编码的核质穿梭蛋白。
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