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[荧光抗体法在肾小球肾炎患者乙型肝炎病毒感染诊断中的应用]

[The fluorescent antibody method in the diagnosis of hepatitis B virus infection in glomerulonephritis patients].

作者信息

Filimonova R G, Nevraeva E G, Posevaia T A, Barinskiĭ I F

出版信息

Ter Arkh. 1990;62(2):116-9.

PMID:2186493
Abstract

In 107 patients with glomerulonephritis (GN) (28 were with acute and 79 with chronic GN) and 54 patients afflicted with other diseases of the kidneys, peripheral blood leukocytes were examined for HBsAg by indirect immunofluorescence. In 60 patients with GN and 32 patients with other diseases, blood sera were examined for HBsAg and anti-HBs by enzyme immunoassay along with examination of blood leukocytes for HBsAg. In blood leukocytes of GN patients, HBsAg was detected in 23.4% of cases, whereas in patients afflicted with other diseases of the kidneys, in 5.6% of cases. In the blood serum, HBsAg was also demonstrated more frequently in GN patients (in 15% of cases) as compared to patients with other diseases of the kidneys (in 3.1% of cases). The rate of anti-HBs demonstration did not significantly differ in patients with GN and in those suffering from other diseases of the kidneys. None of the examined patients both with GN and other diseases of the kidneys showed at a time HBsAg and anti-HBs in the serum or HBsAg in leukocytes and anti-HBs in the blood serum. HBsAg in leukocytes and in the blood serum was identified at a time only in one patient afflicted with chronic GN.

摘要

对107例肾小球肾炎(GN)患者(其中28例为急性肾小球肾炎,79例为慢性肾小球肾炎)和54例患有其他肾脏疾病的患者,采用间接免疫荧光法检测外周血白细胞中的乙肝表面抗原(HBsAg)。对60例GN患者和32例患有其他疾病的患者,采用酶免疫分析法检测血清中的HBsAg和抗-HBs,同时检测血白细胞中的HBsAg。在GN患者的血白细胞中,23.4%的病例检测到HBsAg,而在患有其他肾脏疾病的患者中,这一比例为5.6%。与患有其他肾脏疾病的患者(3.1%的病例)相比,GN患者血清中HBsAg的检出率也更高(15%的病例)。GN患者和患有其他肾脏疾病的患者中抗-HBs的检出率无显著差异。在所有接受检查的GN患者和患有其他肾脏疾病的患者中,均未同时在血清中检测到HBsAg和抗-HBs,或在白细胞中检测到HBsAg且在血清中检测到抗-HBs。仅在1例慢性GN患者中同时在白细胞和血清中检测到HBsAg。

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[The fluorescent antibody method in the diagnosis of hepatitis B virus infection in glomerulonephritis patients].[荧光抗体法在肾小球肾炎患者乙型肝炎病毒感染诊断中的应用]
Ter Arkh. 1990;62(2):116-9.
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