多药耐药相关蛋白4与直肠癌放射敏感性关系的初步研究

[Preliminary study on relationship between multi-drug resistance-associated protein 4 and radiosensitivity of rectal cancer].

作者信息

Chai Rui, Yu Zhi-qi, Fu Chuan-gang, Wang Hao, Zhang Wei, Liu Lian-jie, Zhang Chang

机构信息

Department of Colorectal Surgery, Changhai Hospital, The Second Military Medical University, Shanghai 200433, China.

出版信息

Zhonghua Wei Chang Wai Ke Za Zhi. 2011 Aug;14(8):627-30.

PMID:21866459

Abstract

OBJECTIVE

To explore the correlation between multi-drug resistance-associated protein 4(MRP4) and the sensitivity of rectal cancer to radiation.

METHODS

A total of 95 patients with advanced rectal cancer and received radiation therapy between January 2000 and January 2009. MRP4 and P53 protein expression in the paraffin-embedded specimen were detected by immunohistochemistry. Logistic regression analysis was used to evaluate factors associated with the sensitivity of rectal cancer to radiation.

RESULTS

Forty patients(42%) were sensitive to radiation therapy, of whom 10(11%) achieved pathological complete remission. Fifty-five patients were (58%) not responsive to radiation. Patients with low expression of MRP4 had a 66.7%(24/36) response rate, significantly higher than that of patients with high MRP4 expression (29.1%,16/59)(P<0.05). Patients with low expression of P53 had a 63.9%(23/36) response rate, significantly higher than that of patients with high P53 expression(28.8%,17/59)(P<0.01). The response rate after long course radiation therapy was 83.3%(20/24), significantly higher than that of patients who underwent short and medium course radiation(31.3%, 5/16) and(27.3%,15/55). Multivariate Logistic regression analysis showed radiation regimen, the expression of P53 and MRP4 protein were independently associated with the sensitivity of rectal cancer to radiation(P<0.05).

CONCLUSION

MRP4 may serve as a predictive marker for the sensitivity of rectal cancer to preoperative radiation.

摘要

目的

探讨多药耐药相关蛋白4（MRP4）与直肠癌放疗敏感性之间的相关性。

方法

选取2000年1月至2009年1月期间95例接受放疗的晚期直肠癌患者。采用免疫组织化学法检测石蜡包埋标本中MRP4和P53蛋白的表达。采用Logistic回归分析评估与直肠癌放疗敏感性相关的因素。

结果

40例（42%）患者对放疗敏感，其中10例（11%）达到病理完全缓解。55例（58%）患者对放疗无反应。MRP4低表达患者的有效率为66.7%（24/36），显著高于MRP4高表达患者（29.1%，16/59）（P<0.05）。P53低表达患者的有效率为63.9%（23/36），显著高于P53高表达患者（28.8%，17/59）（P<0.01）。长程放疗后的有效率为83.3%（20/24），显著高于短程和中程放疗患者[（31.3%，5/16）和（27.3%，15/55）]（P<0.01）。多因素Logistic回归分析显示，放疗方案、P53和MRP4蛋白表达与直肠癌放疗敏感性独立相关（P<0.05）。