Nematology Lab., Department of Plant Protection, Faculty of Agriculture, University of Tabriz, Tabriz, Iran.
J Insect Sci. 2011;11:75. doi: 10.1673/031.011.7501.
Hemocyte encapsulation reactions of infective juveniles of two Iranian isolates of the entomopathogenic nematodes, Heterorhabditis bacteriophora Poinar (Rhabditina: Heterorhabditidae) and Steinernema feltiae Filipjev (Tylenchina: Steinernematidae), were compared in the economic pest Colorado potato beetle, Leptinotarsa decemlineata Say (Coleoptera: Chrysomelidae), and the greater wax moth, Galleria mellonella L. (Lepidoptera: Pyralidae). The former was a more responsive host than the latter and the hemocyte responses occurred sooner and more extensively. Complete encapsulation of some of the nematodes occurred by 4 h post injection for H. bacteriophora in both L. decemlineata and G. mellonella, and by 2 h pi for S. feltiae in L. decemlineata. The percentage of encapsulation from 24 h to 72 h pi in L. decemlineata was 86.2% for S. feltiae and 39% for H. bacteriophora. In G. mellonella there were no encapsulation or melanization responses against S. feltiae, whereas when H. bacteriophora was encapsulated and melanized (16.7%) the encapsulation level was lower than in L. decemlineata. This study may contribute to effectively selecting entomopathogenic nematode species active against significant economic pests based on the latter's cellular immune response.
在经济害虫——马铃薯甲虫（鞘翅目：叶甲科）和大蜡螟（鳞翅目：螟蛾科）中，比较了两种伊朗来源的昆虫病原线虫——嗜菌异小杆线虫（小杆目：异小杆科）和斯氏线虫（垫刃目：斯氏科）的侵染期幼虫的血细胞包埋反应。前者比后者更敏感，血细胞反应发生得更早、更广泛。在马铃薯甲虫和大蜡螟中，一些线虫在注射后 4 小时内被完全包埋，而在马铃薯甲虫中，斯氏线虫在 2 小时内被包埋。在马铃薯甲虫中，24 至 72 小时的包埋率分别为斯氏线虫 86.2%和嗜菌异小杆线虫 39%。在大蜡螟中，没有针对斯氏线虫的包埋或黑化反应，而当嗜菌异小杆线虫被包埋和黑化（16.7%）时，其包埋水平低于马铃薯甲虫。本研究可能有助于根据后者的细胞免疫反应，有效地选择对重要经济害虫有活性的昆虫病原线虫。
