Department of Biology, University of Padova, Via Ugo Bassi 58/B, 35131 Padova, Italy.
Mar Pollut Bull. 2011 Oct;62(10):2042-6. doi: 10.1016/j.marpolbul.2011.07.025. Epub 2011 Aug 24.
The effects of nonylphenol (NP) on functional responses of haemocytes of the colonial ascidian Botryllus schlosseri were evaluated. Haemocytes were exposed to 0.1, 1 and 10 μM NP and the effects on haemocyte viability, adhesion, morphology, lysosomal membrane stability, phagocytic activity and early apoptosis were evaluated. Haemocyte viability and adhesion were not affected by NP. Phagocytic activity and the amoebocytic index decreased significantly at all the concentrations tested, while exposure of haemocytes to 1 and 10 μM NP caused a significant increase in the diffusion of Neutral Red into the cytosol. The percentage of cells positive to Annexin-V (indicative of early apoptosis) increased significantly at 1 and 10 μM NP. Results obtained suggested a relationship between NP and alterations in functional responses of haemocytes in B. schlosseri. Biomarkers measured resulted sensitive, rapid and reproducible, even if their responsiveness will be evaluated after in vivo exposure of animals to NP.
评估了壬基酚 (NP) 对群体海鞘 Botryllus schlosseri 血细胞功能反应的影响。将血细胞暴露于 0.1、1 和 10 μM NP，并评估其对血细胞活力、黏附、形态、溶酶体膜稳定性、吞噬活性和早期凋亡的影响。NP 对血细胞活力和黏附没有影响。在所有测试浓度下，吞噬活性和变形细胞指数均显著降低，而血细胞暴露于 1 和 10 μM NP 会导致中性红向细胞质扩散显著增加。 Annexin-V 阳性细胞的百分比（早期凋亡的指示）在 1 和 10 μM NP 时显著增加。结果表明 NP 与 B. schlosseri 血细胞功能反应的改变之间存在关系。测量的生物标志物灵敏、快速且重现性好，即使在动物体内暴露于 NP 后，也将评估其反应性。
