Kondratyev A D, Popova O N, Severin S E, Choladze M A, Shmyrev I I, Tubasheva I A, Zotova E E, Posypanova G A, Severin E S
Research Center of Molecular Diagnostics, USSR Ministry of Health, Moscow.
FEBS Lett. 1990 May 7;264(1):75-7. doi: 10.1016/0014-5793(90)80768-e.
The effect of nerve growth factor (NGF) on the activity and intracellular localization of protein kinase C (PKC) in pheochromocytoma PC12 cells was studied. By using immunoblotting, immunofluorescence method and phorbol ester binding, NGF was found to induce PKC translocation from the cytoplasm into the cell membrane. This process was accompanied by an increase in the protein kinase activity in the membrane fraction. Translocation was completely blocked by H-7, a protein kinase inhibitor potentiating the neurite-stimulating activity of NGF.
研究了神经生长因子（NGF）对嗜铬细胞瘤PC12细胞中蛋白激酶C（PKC）活性及细胞内定位的影响。通过免疫印迹法、免疫荧光法和佛波酯结合实验发现，NGF可诱导PKC从细胞质转位至细胞膜。这一过程伴随着膜组分中蛋白激酶活性的增加。转位被蛋白激酶抑制剂H-7完全阻断，H-7可增强NGF的神经突刺激活性。
