Shanab Sanaa M M, Shalaby Emad A, El-Fayoumy Eman A
Botany Department, Faculty of Science, Cairo University, Giza 12613, Egypt.
J Biomed Biotechnol. 2011;2011:726405. doi: 10.1155/2011/726405. Epub 2011 Aug 14.
The green macroalgae, Enteromorpha compressa (Linnaeus) Nees, Ulva lactuca, and E. linza, were seasonally collected from Abu Qir bay at Alexandria (Mediterranean Sea) This work aimed to investigate the seasonal environmental conditions, controlling the green algal growth, predominance, or disappearance and determining antioxidant activity. The freshly collected selected alga (E. compressa) was subjected to pigment analysis (chlorophyll and carotenoids) essential oil and antioxidant enzyme determination (ascorbate oxidase and catalase). The air-dried ground alga was extracted with ethanol (crude extract) then sequentially fractionated by organic solvents of increasing polarity (petroleum ether, chloroform, ethyl acetate, and water). Antioxidant activity of all extracts was assayed using different methods (total antioxidant, DPPH [2, 2 diphenyl-1-picrylhydrazyl], ABTS [2, 2 azino-bis ethylbenzthiazoline-6-sulfonic acid], and reducing power, and β-carotene linoleic acid bleaching methods). The results indicated that the antioxidant activity was concentration and time dependent. Ethyl acetate fraction demonstrated higher antioxidant activity against DPPH method (82.80%) compared to the synthetic standard butylated hydroxyl toluene (BHT, 88.5%). However, the crude ethanolic extract, pet ether, chloroform fractions recorded lower to moderate antioxidant activities (49.0, 66.0, and 78.0%, resp.). Using chromatographic and spectroscopic analyses, an active compound was separated and identified from the promising ethyl acetate fraction.
绿色大型海藻，皱波角叉菜（林奈）内斯、石莼和缘管浒苔，是从亚历山大港（地中海）的阿布吉尔湾季节性采集的。这项工作旨在调查季节性环境条件，控制绿藻的生长、优势或消失情况，并测定其抗氧化活性。对新鲜采集的选定藻类（皱波角叉菜）进行色素分析（叶绿素和类胡萝卜素）、精油和抗氧化酶测定（抗坏血酸氧化酶和过氧化氢酶）。将风干磨碎的藻类用乙醇提取（粗提物），然后依次用极性递增的有机溶剂（石油醚、氯仿、乙酸乙酯和水）进行分级分离。使用不同方法（总抗氧化剂、DPPH[2,2-二苯基-1-苦基肼]、ABTS[2,2'-联氮-双-（3-乙基苯并噻唑啉-6-磺酸）]、还原能力和β-胡萝卜素亚油酸漂白法）测定所有提取物的抗氧化活性。结果表明，抗氧化活性具有浓度和时间依赖性。与合成标准丁基羟基甲苯（BHT，88.5%）相比，乙酸乙酯馏分对DPPH法表现出更高的抗氧化活性（82.80%）。然而，乙醇粗提物、石油醚、氯仿馏分的抗氧化活性较低至中等（分别为49.0%、66.0%和78.0%）。通过色谱和光谱分析，从有前景的乙酸乙酯馏分中分离并鉴定出一种活性化合物。
