Shanghai Key Laboratory of Functional Materials Chemistry, and Research Center of Analysis and Test, East China University of Science and Technology , Shanghai , P.R. China.
Pharm Biol. 2013 Nov;51(11):1419-25. doi: 10.3109/13880209.2013.794844. Epub 2013 Jul 10.
Alpinia oxyphylla Miquel (Zingiberaceae) is a traditional Chinese herbal medicine widely used for the treatment of intestinal disorders, urosis and diuresis. However, information about antioxidant and cytotoxic properties of its fruits remains to be elucidated.
The ethanol crude extract (CE) and its fractions [petroleum ether fraction (PF), ethyl acetate fraction (EF), n-butanol fraction (BF) and water fraction (WF) extracted by petroleum ether, ethyl acetate, n-butanol and water, respectively] of A. oxyphylla fruits were investigated for their antioxidant activity and cytotoxicity.
The total phenolic content (TPC) and antioxidant activity of the extracts were determined by Folin-Ciocalteu reagent, 1,1-diphenyl-2-picrylhydrazyl (DPPH(•)), Trolox equivalent antioxidant capacity and reducing power assay. Cytotoxicity of the extracts (0-200 μg/mL) was tested on six human cancer cell lines (breast cancer cell line, cervix carcinoma cell line, lung adenocarcinoma cell line, liver carcinoma cell line, gastric cancer cell line and colon cancer cell line) using the sulforhodamine B assay.
The TPC of extracts varied from 8.2 to 20.3 mg gallic acid equivalents/g dry weight. DPPH radical scavenging effect of extracts decreased in the order of EF > BF > CE > PF > WF, with IC50 values ranging from 74.7 to 680.8 μg/mL. 2,2-azo-bis(3-Ethylbenzothiazoline-6-sulfoic acid) diammonium salt scavenging activity ranged from 0.118 to 0.236 mmol Trolox equivalence/mg extract. The extracts exhibited concentration-dependent reducing power, and EF showed the highest reducing ability. A satisfactory correlation (R(2) > 0.826) between TPC and antioxidant activity was observed. In addition, EF, PF and CE exhibited potent anticancer effects on six cancer cell lines with IC50 values ranging from 40.1 to 166.3 μg/mL.
The ethanol extract of A. oxyphylla fruit, especially the EF, was found to possess potent antioxidant and anticancer activities, and thus a great potential for the application in food and drug products.
益智（姜科）是一种传统的中草药，广泛用于治疗肠道疾病、尿路病和利尿。然而，其果实的抗氧化和细胞毒性特性的信息尚待阐明。
研究益智果实的乙醇粗提取物（CE）及其各部分[分别用石油醚、乙酸乙酯、正丁醇和水提取的石油醚部分（PF）、乙酸乙酯部分（EF）、正丁醇部分（BF）和水部分（WF）]的抗氧化活性和细胞毒性。
用福林-肖卡试剂、1,1-二苯基-2-苦基肼（DPPH(•)）、Trolox 当量抗氧化能力和还原力测定法测定提取物的总酚含量（TPC）和抗氧化活性。用磺基罗丹明 B 测定法在六种人癌细胞系（乳腺癌细胞系、宫颈癌细胞系、肺腺癌细胞系、肝癌细胞系、胃癌细胞系和结肠癌细胞系）上测试提取物（0-200μg/mL）的细胞毒性。
提取物的 TPC 从 8.2 到 20.3mg 没食子酸当量/g 干重不等。提取物对 DPPH 自由基的清除作用依次为 EF>BF>CE>PF>WF，IC50 值范围为 74.7 到 680.8μg/mL。2,2-偶氮-双（3-乙基苯并噻唑啉-6-磺酸）二铵盐清除活性为 0.118 到 0.236mmol Trolox 当量/mg 提取物。提取物表现出浓度依赖性的还原能力，EF 表现出最强的还原能力。观察到 TPC 与抗氧化活性之间存在令人满意的相关性（R(2)>0.826）。此外，EF、PF 和 CE 对六种癌细胞系表现出强大的抗癌作用，IC50 值范围为 40.1 到 166.3μg/mL。
益智果实的乙醇提取物，特别是 EF，具有强大的抗氧化和抗癌活性，因此在食品和药物产品中有很大的应用潜力。
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