Homer Hayden
Mammalian Oocyte and Embryo Research Laboratory, Division of Biosciences and Institute for Women's Health, University College London, London, UK.
Methods Mol Biol. 2011;782:33-45. doi: 10.1007/978-1-61779-273-1_4.
The spindle assembly checkpoint (SAC) is a quality control mechanism for overseeing the fidelity of chromosome segregation. By modulating the activity of the anaphase-promoting complex or cyclosome (APC/C), the SAC sets the timing of anaphase-onset by co-ordinating the timely destruction of key proteins with the completion of chromosome alignment. How mammalian oocytes regulate chromosome segregation during the first meiotic division (meiosis I) is of immense importance as mis-segregation at this crucial stage in human oocytes underpins the majority of human aneuploidy and birth defects. In recent years, the SAC has been shown to be indispensable for the accuracy of meiosis I chromosome segregation. Here, I describe a technique based on immunoblotting for evaluating SAC competence during meiosis I in mouse oocytes.
纺锤体组装检查点（SAC）是一种用于监督染色体分离保真度的质量控制机制。通过调节后期促进复合体或周期体（APC/C）的活性，SAC通过协调关键蛋白质的及时降解与染色体排列的完成来设定后期开始的时间。哺乳动物卵母细胞在第一次减数分裂（减数分裂I）期间如何调节染色体分离至关重要，因为人类卵母细胞在这个关键阶段的错误分离是大多数人类非整倍体和出生缺陷的基础。近年来，SAC已被证明对于减数分裂I染色体分离的准确性不可或缺。在这里，我描述了一种基于免疫印迹的技术，用于评估小鼠卵母细胞减数分裂I期间的SAC能力。
