Iurinskaia V E, Rubashkin A A, Shirokova A V, Vereninov A A
Tsitologiia. 2011;53(6):474-81.
Time course of changes in intracellular water, K+ and Na+ of U937 cells incubated in hyperosmolar medium with addition of 200 mM sucrose was studied. Ouabain-sensitive and ouabain-resistant Rb+ (K+) influxes were measured during regulatory cell volume increase (RVI) and apoptotic volume decrease (AVD). Microscopy of cells stained by Acrydine orange, Ethydium bromide, APOPercenrage Dye and polycaspase marker FLICA was performed. We found that initial osmotic cell shrinkage induced both RVI and AVD responses. RVI dominated at the early stage whereas AVD prevailed at the later stage. In view of the data obtained in U937 cells the current opinion that RVI "dysfunction" is a prerequisite for apoptosis and AVD (Subramanyam et al., 2010) should be revised. U937 cells are capable to trigger of apoptosis and AVD in spite of the unimpaired RVI response. It is concluded that AVD plays a significant role in preventing osmotic lysis of apoptotic cells rather than in the initiation of apoptosis.
研究了在添加200 mM蔗糖的高渗培养基中培养的U937细胞内水、K⁺和Na⁺的变化时间进程。在调节性细胞容积增加（RVI）和凋亡性容积减少（AVD）过程中测量了哇巴因敏感和哇巴因抵抗的Rb⁺（K⁺）流入。对用吖啶橙、溴化乙锭、APOPercenrage染料和多聚半胱天冬酶标记物FLICA染色的细胞进行了显微镜检查。我们发现初始渗透性细胞收缩诱导了RVI和AVD反应。RVI在早期占主导，而AVD在后期占优势。鉴于在U937细胞中获得的数据，当前认为RVI“功能障碍”是凋亡和AVD的先决条件的观点（Subramanyam等人，2010年）应予以修正。尽管RVI反应未受损，U937细胞仍能够触发凋亡和AVD。得出的结论是，AVD在防止凋亡细胞的渗透性溶解中起重要作用，而不是在凋亡的起始中起作用。
