Hofmann K H, Neubauer P, Riethdorf S, Hecker M
Sektion Biologie, Ernst-Moritz-Arndt-Universität, Griefswald, DDR.
J Basic Microbiol. 1990;30(1):37-41. doi: 10.1002/jobm.3620300111.
Fermenter studies under batch and fed-batch conditions were carried out to test the possibility of plasmid pBR322 production in large amounts by using E. coli relA strains. High amplification rates of pBR322 plasmid DNA were observed in E. coli CP79 (relA) and E. coli CP143 (relA) in both batch and fed-batch cultivation after exhaustion of the amino acid arginine. The concentrations of plasmid DNA per unit of biomass were nearly the same in batch and in fed-batch fermentations of E. coli CP79 and E. coli CP143. Therefore, the significantly higher biomass concentration of the two strains after fed-batch fermentation gave a dramatic increase in the yield of plasmid DNA per litre of medium in comparison to the batch process. The results support the suggestion that E. coli relA strains are suitable hosts for production of large amounts of ColE1-derived plasmids for recombinant DNA research.
在分批和补料分批条件下进行了发酵罐研究，以测试使用大肠杆菌relA菌株大量生产质粒pBR322的可能性。在精氨酸耗尽后的分批和补料分批培养中，在大肠杆菌CP79（relA）和大肠杆菌CP143（relA）中观察到pBR322质粒DNA的高扩增率。在大肠杆菌CP79和大肠杆菌CP143的分批和补料分批发酵中，每单位生物量的质粒DNA浓度几乎相同。因此，与分批过程相比，补料分批发酵后两株菌显著更高的生物量浓度使每升培养基中质粒DNA的产量大幅增加。结果支持了这样的建议，即大肠杆菌relA菌株是用于重组DNA研究的大量生产ColE1衍生质粒的合适宿主。
