Oliver G, Novak S, Patterson J L, Pasternak J A, Paradis F, Norrby M, Oxtoby K, Dyck M K, Dixon W T, Foxcroft G R
Department of Agricultural, Food and Nutritional Science, University of Alberta, Edmonton, AB, Canada.
Reprod Fertil Dev. 2011;23(7):899-911. doi: 10.1071/RD11013.
Expression of panels of candidate genes controlling myogenesis, angiogenesis and gender-specific imprinting of development were analysed in embryonic, placental and endometrial tissues recovered at Day 30 of gestation from a subset of primiparous sows that were either feed restricted (Restrict; n=17) or fed to appetite (Control; n=15) during the last week of the previous lactation. Embryos were also sex typed to investigate gender bias in response to treatments. Average embryonic weight was lower in the subset of Restrict compared with Control litters (1.38±0.07vs 1.59±0.08g, respectively) and the male:female sex ratio was higher (P<0.05) in embryos (litters) recovered from Restrict sows. Treatment affected (P≤0.05) the expression of embryonic and placental genes involved in insulin-like growth factor (IGF) 2 signalling, including IGF2, INSR and IGF2R. Embryonic expression of ESR1 was also affected by treatment (P<0.03) and sex×treatment interactions were observed for the expression of embryonic ESR1 (P<0.05) and placental ANGPT2 (P<0.03). At the molecular level, these results support the suggestion that changes in placental function are not the primary mechanism mediating detrimental effects of previous sow catabolism on early embryonic development in the feed-restricted lactational sow model. However, perturbations in the IGF2 system are implicated as mediators of these effects.
对一组控制肌生成、血管生成和发育性别特异性印记的候选基因在妊娠第30天从初产母猪的一个亚组中回收的胚胎、胎盘和子宫内膜组织中的表达进行了分析。这些初产母猪在前次泌乳的最后一周要么限饲（限饲组；n = 17），要么自由采食（对照组；n = 15）。还对胚胎进行了性别鉴定，以研究处理反应中的性别偏差。限饲组仔猪的平均胚胎重量低于对照组（分别为1.38±0.07克和1.59±0.08克），从限饲母猪回收胚胎（仔猪）中的雄雌性别比更高（P<0.05）。处理影响（P≤0.05）参与胰岛素样生长因子（IGF）2信号传导的胚胎和胎盘基因的表达，包括IGF2、INSR和IGF2R。ESR1的胚胎表达也受处理影响（P<0.03），并且观察到胚胎ESR1表达的性别×处理交互作用（P<0.05）和胎盘ANGPT2表达的性别×处理交互作用（P<0.03）。在分子水平上，这些结果支持以下观点：在限饲泌乳母猪模型中，胎盘功能的变化不是介导先前母猪分解代谢对早期胚胎发育有害影响的主要机制。然而，IGF2系统的扰动被认为是这些影响的介质。
