Department of Therapeutic Research and Medicines Evaluation, Istituto Superiore di Sanità, V.le Regina Elena 299, 00161 Rome, Italy.
J Pharm Biomed Anal. 2011 Dec 15;56(5):1112-6. doi: 10.1016/j.jpba.2011.07.046. Epub 2011 Aug 4.
A simple and rapid gas chromatography (GC) method with mass spectrometry (MS) detection has been developed for the determination of methyldibromo glutaronitrile (MDBGN) in cosmetic products. The presence of this preservative in commercial cosmetic samples is prohibited since 2007 because of its allergenic properties. The analyzed products were opportunely diluted in methanol and MDBGN was separated by fused silica capillary column and detected by electron impact (EI)-MS in positive ionization mode with a total run time of 7 min. The assay was validated in the range 0.005-0.100 mg MDBGN per g of examined product with good determination coefficients (r(2)≥0.99) for the calibration curves. At three concentrations spanning the linear dynamic range of the calibration curves, mean recoveries were always higher than 95% for MDBGN in the tested cosmetics. This method was successfully applied to the analysis of cleansing gels, shampoo and solar waters to disclose the eventual presence of MDBGN illegally added in cosmetic products.
建立了一种简单、快速的气相色谱（GC）-质谱（MS）检测法，用于检测化妆品中的甲二溴戊二腈（MDBGN）。自 2007 年以来，由于其变应原特性，这种防腐剂已被禁止在商业化妆品样品中使用。分析的产品在甲醇中适当稀释，MDBGN 用熔融石英毛细管柱分离，在正离子化模式下用电离（EI）-MS 检测，总运行时间为 7 分钟。该测定法在 0.005-0.100mgMDBGN/g 被检产品的范围内进行了验证，校准曲线的相关系数（r（2）≥0.99）良好。在跨越校准曲线线性动态范围的三个浓度下，MDBGN 在测试化妆品中的平均回收率始终高于 95%。该方法成功应用于清洁凝胶、洗发水和防晒霜的分析，以揭示化妆品中非法添加 MDBGN 的情况。
