US Food and Drug Administration, Center for Food Safety and Applied Nutrition, 5100 Paint Branch Parkway, HFS-717, College Park, MD 20740-3835, USA.
J Chromatogr B Analyt Technol Biomed Life Sci. 2011 Jun 15;879(20):1795-801. doi: 10.1016/j.jchromb.2011.04.030. Epub 2011 May 6.
A rapid method for the determination of para-phenylenediamine (PPD) in cosmetic products, such as henna tattoos has been developed and evaluated. This analytical procedure involved extracting a 10mg test portion of cosmetic product in 10 mL of ethyl acetate, followed by determination by gas chromatography-mass spectrometry in the selected ion monitoring mode (GC/MS-SIM). 1,4-Phenylenediamine-2,3,5,6-d(4) was selected as an internal standard that was added at the beginning of the extraction procedure and used to correct for recovery and matrix effects. The linearity ranged from 1.0 to 1275 μg/mL with a coefficient of determination (r(2)) greater than 0.999. LOQ and LOD were 1.0 and 0.10 μg/mL, respectively. The recovery in a tattoo product containing PPD was 94% and that for a tattoo product containing no PPD reached 105%. Extraction efficiency of 98% was obtained. This method has been successfully applied to henna temporary tattoo and other henna-related cosmetic products for the determination and quantitation of PPD.
一种快速测定化妆品中对苯二胺（PPD）的方法，如指甲花纹身，已经建立并评估。该分析程序包括在 10mL 乙酸乙酯中提取 10mg 化妆品测试部分，然后在选择离子监测模式下（GC/MS-SIM）进行气相色谱-质谱法测定。1,4-苯二胺-2,3,5,6-d(4)被选为内标，在提取程序开始时加入，用于校正回收率和基质效应。线性范围为 1.0 至 1275μg/mL，相关系数（r(2)）大于 0.999。LOQ 和 LOD 分别为 1.0 和 0.10μg/mL。含 PPD 的纹身产品的回收率为 94%，不含 PPD 的纹身产品的回收率为 105%。提取效率为 98%。该方法已成功应用于指甲花临时纹身和其他指甲花相关化妆品产品中 PPD 的测定和定量。
