Institut für Biochemie der Universität Stuttgart, Stuttgart, Germany.
FEBS Lett. 2011 Oct 3;585(19):3015-9. doi: 10.1016/j.febslet.2011.08.021. Epub 2011 Aug 23.
The endoplasmic reticulum (ER) is responsible for folding and delivery of secretory proteins to their site of action. One major modification proteins undergo in this organelle is N-glycosylation. Proteins that cannot fold properly will be directed to a process known as endoplasmic reticulum associated degradation (ERAD). Processing of N-glycans generates a signal for ERAD. The lectin Yos9 recognizes the N-glycan signal of misfolded proteins and acts as a gatekeeper for the delivery of these substrates to the cytoplasm for degradation. Presence of Yos9 accelerates degradation of the glycosylated model ERAD substrate CPY∗. Here we show that Yos9 has also a control function in degradation of the unglycosylated ERAD substrate CPY∗0000. It decelerates its degradation rate.
内质网（ER）负责将分泌蛋白折叠并递送到其作用部位。该细胞器中蛋白质经历的主要修饰之一是 N-糖基化。不能正确折叠的蛋白质将被定向到内质网相关降解（ERAD）过程中。N-聚糖的加工会产生 ERAD 的信号。凝集素 Yos9 识别错误折叠蛋白质的 N-聚糖信号，并作为将这些底物递送到细胞质进行降解的门控蛋白。Yos9 的存在加速了糖基化的 ERAD 底物 CPY∗的降解。在这里，我们表明 Yos9 还在未糖基化的 ERAD 底物 CPY∗0000 的降解中具有控制功能。它会降低其降解速度。
