The Adams Ann Department of Pediatrics, Wayne State University School of Medicine, Detroit, MI 48201, USA.
Environ Res. 2011 Nov;111(8):1215-21. doi: 10.1016/j.envres.2011.08.003. Epub 2011 Aug 27.
Understanding the interplay between genes and in-utero tobacco exposure in affecting child lung development is of great significance. In this study, we tested the hypothesis that tobacco-related lung-function reduction in children differs by maternal polymorphic genes Cytochrome P450 1A1 (CYP1A1) and Glutathione S-transferase Mu 1 (GSTM1).
Data were collected among 370 children (6-10 years old, 81.6% African-Americans) and their biological mothers visiting a large children's hospital. Study hypotheses were tested using multiple regression method.
Among the study sample, 143 mothers smoked throughout pregnancy and 72 smoked on a daily basis. Spirometric measures (mean±SD) included were: forced vital capacity (FVC)=1635±431 mL, forced expiratory volume in the first 1s (FEV1)=1440 ±360 mL, percent FEV1/FVC ratio=89±12, and forced expiratory flow between the 25% and 75% of FVC (FEF25-75)=1745±603 mL. In addition to a tobacco effect on FVC (-131 mL, 95% CI: -245, -17) and FEV1/FVC ratio (42, 95% CI: 1, 83), regression analysis controlling for covariates indicated that for the subsample of children whose mothers were CYP1A1⁎2A homozygous, maternal daily smoking was associated with -734 mL (95% CI: -1206, -262) reductions in FEV1 and -825 mL (95% CI: -909, -795) reductions in FVC; reduced smoking was still associated with -590 mL (95% CI: -629, -551) reductions in FVC. For children of mothers with GSTM1 deletion, persistent daily smoking was associated with -176 mL (95% CI: -305, -47) reductions in FVC.
Maternal smoking during pregnancy was significantly associated with lung-function reduction in children, particularly for those whose mothers possessed the polymorphic CYP1A1*2A and GSTM1 deletion.
了解基因与子宫内烟草暴露在影响儿童肺发育方面的相互作用非常重要。在这项研究中，我们检验了这样一个假设，即与烟草相关的儿童肺功能下降因母亲多态性基因细胞色素 P450 1A1（CYP1A1）和谷胱甘肽 S-转移酶 Mu 1（GSTM1）而异。
数据收集于 370 名儿童（6-10 岁，81.6%为非裔美国人）及其在一家大型儿童医院就诊的生物母亲。使用多元回归法检验研究假设。
在研究样本中，有 143 位母亲在整个怀孕期间吸烟，有 72 位母亲每天吸烟。检测到的肺功能指标（均值±标准差）包括：用力肺活量（FVC）=1635±431 毫升，第 1 秒用力呼气量（FEV1）=1440±360 毫升，FEV1/FVC 比值=89±12，用力呼出 25%至 75%肺活量之间的流量（FEF25-75）=1745±603 毫升。除了烟草对 FVC（-131 毫升，95%CI：-245，-17）和 FEV1/FVC 比值（42，95%CI：1，83）的影响外，控制协变量的回归分析表明，对于其母亲 CYP1A1*2A 纯合子的子样本，母亲每天吸烟与 FEV1 减少 734 毫升（95%CI：-1206，-262）和 FVC 减少 825 毫升（95%CI：-909，-795）相关；减少吸烟仍与 FVC 减少 590 毫升（95%CI：-629，-551）相关。对于母亲携带 GSTM1 缺失的儿童，持续每日吸烟与 FVC 减少 176 毫升（95%CI：-305，-47）相关。
母亲怀孕期间吸烟与儿童肺功能下降显著相关，尤其是对于其母亲携带多态性 CYP1A1*2A 和 GSTM1 缺失的儿童。
