Department of Urology, the First Affiliated Hospital of China Medical University, Shenyang, China.
Urol Oncol. 2013 Feb;31(2):228-33. doi: 10.1016/j.urolonc.2010.11.011. Epub 2011 Aug 26.
To uncover the role of c-Jun, a proto-oncogene, in inhibitory effects of antiproliferative factor (APF) on tumor cell growth.
Expression of c-Jun was analyzed by Western blotting in 45 clinical specimens (30 tumorous tissues and 15 paired non-tumorous tissues) and 3 bladder cancer cell lines. APF-responsive T24 transitional carcinoma bladder cells were treated with APF or mock control. Cell proliferation was measured by 3-(4,5-dimethylthiazolyl-2)-2,5-diphenyltetrazolium bromide (MTT) assay. Change of c-Jun expression was detected by RT-PCR and Western blotting. The influence of c-Jun on APF treatment was evaluated by transient transfection of c-Jun and MTT assay in T24 cells.
c-Jun was significantly higher in invasive bladder cancer tissues and cell lines. T24 cells treated with APF had decreased c-Jun expression and suppressed cell growth. More importantly, ectopic c-Jun attenuated APF inhibitory effects on cell growth.
These observations suggest that c-Jun is involved in APF-mediated inhibition for bladder tumor cell growth, as potential target of APF in patients with aggressive bladder carcinoma.
揭示原癌基因 c-Jun 在增殖抑制因子 (APF) 抑制肿瘤细胞生长中的作用。
通过 Western blot 分析 45 例临床标本（30 例肿瘤组织和 15 例配对非肿瘤组织）和 3 株膀胱癌细胞系中 c-Jun 的表达。用 APF 或模拟对照处理对 APF 有反应的 T24 移行细胞膀胱癌细胞。通过 3-(4,5-二甲基噻唑-2)-2,5-二苯基四氮唑溴盐 (MTT) 测定法测量细胞增殖。通过 RT-PCR 和 Western blot 检测 c-Jun 表达的变化。通过瞬时转染 c-Jun 和 MTT 测定法在 T24 细胞中评估 c-Jun 对 APF 治疗的影响。
侵袭性膀胱癌组织和细胞系中 c-Jun 的表达明显升高。用 APF 处理的 T24 细胞 c-Jun 表达减少，细胞生长受到抑制。更重要的是，外源性 c-Jun 减弱了 APF 对细胞生长的抑制作用。
这些观察结果表明，c-Jun 参与了 APF 介导的膀胱肿瘤细胞生长抑制，作为侵袭性膀胱癌患者中 APF 的潜在靶点。
