Department of Environment and Energy, Sejong University, Seoul 143-747, South Korea.
Chemosphere. 2011 Nov;85(8):1360-7. doi: 10.1016/j.chemosphere.2011.08.001. Epub 2011 Aug 27.
Microbial degradation-induced changes in the characteristics of dissolved organic matter (DOM), and the subsequent effects on phenanthrene-DOM interactions were investigated based on the microbial incubation of DOM collected from four different sources for 28 d. Partially biodegraded DOM presented higher specific UV absorbance (SUVA), lower protein-like fluorescence, higher humic-like fluorescence, lower aliphatic carbon fraction, and higher hydrophobic neutral fractions compared to the original DOM. Microbial changes in DOM led to an increase in the isotherm nonlinearity as well as the extent of phenanthrene binding. A negative relationship between SUVA and the Freundlich n values was established for the original and the biodegraded DOM, suggesting that aromatic condensed structures may play important roles in providing nonlinear strong binding sites irrespective of microbial degradation. In contrast, there were two separate slopes of the correlations between the percentage of hydrophobic acid (HoA) fraction and the n values for the original and the biodegraded DOM with a higher slope exhibited for the latter, implying that the microbial utilization of oxygen-containing structures in the HoA fractions may contribute to enhancing the associated isotherm nonlinearity.
基于对四种不同来源的 DOM 进行 28 天微生物培养，研究了微生物降解诱导的溶解有机物质（DOM）特性变化，以及随后对菲-DOM 相互作用的影响。部分生物降解的 DOM 与原始 DOM 相比，具有更高的特定紫外吸光度（SUVA）、更低的蛋白质样荧光、更高的腐殖质样荧光、更低的脂肪碳部分和更高的疏水性中性部分。DOM 中的微生物变化导致等温线非线性以及菲结合程度增加。对于原始和生物降解的 DOM，SUVA 与 Freundlich n 值之间建立了负相关关系，这表明芳香缩合结构可能在提供非线性强结合位点方面发挥重要作用，而与微生物降解无关。相比之下，原始和生物降解的 DOM 中疏水性酸（HoA）部分的百分比与 n 值之间的相关性存在两个不同的斜率，对于后者斜率更高，这意味着 HoA 部分中含氧结构的微生物利用可能有助于增强相关的等温线非线性。
